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Reprogramming homing endonuclease specificity through computational design and directed evolution

Homing endonucleases (HEs) can be used to induce targeted genome modification to reduce the fitness of pathogen vectors such as the malaria-transmitting Anopheles gambiae and to correct deleterious mutations in genetic diseases. We describe the creation of an extensive set of HE variants with novel...

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Detalles Bibliográficos
Autores principales:	Thyme, Summer B., Boissel, Sandrine J. S., Arshiya Quadri, S., Nolan, Tony, Baker, Dean A., Park, Rachel U., Kusak, Lara, Ashworth, Justin, Baker, David
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936771/ https://www.ncbi.nlm.nih.gov/pubmed/24270794 http://dx.doi.org/10.1093/nar/gkt1212

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