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Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona
BACKGROUND: Encased in a matrix of extracellular polymeric substances (EPS) composed of flagella, adhesins, amyloid fibers (curli), and exopolysaccharides (cellulose, β-1,6-N-acetyl-D-glucosamine polymer-PGA-, colanic acid), the bacteria Escherichia coli is able to attach to and colonize different t...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936827/ https://www.ncbi.nlm.nih.gov/pubmed/24568619 http://dx.doi.org/10.1186/1756-0500-7-108 |
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author | Gómez-Gómez, José María Amils, Ricardo |
author_facet | Gómez-Gómez, José María Amils, Ricardo |
author_sort | Gómez-Gómez, José María |
collection | PubMed |
description | BACKGROUND: Encased in a matrix of extracellular polymeric substances (EPS) composed of flagella, adhesins, amyloid fibers (curli), and exopolysaccharides (cellulose, β-1,6-N-acetyl-D-glucosamine polymer-PGA-, colanic acid), the bacteria Escherichia coli is able to attach to and colonize different types of biotic and abiotic surfaces forming biofilms and colonies of intricate morphological architectures. Many of the biological aspects that underlie the generation and development of these E. coli’s formations are largely poorly understood. RESULTS: Here, we report the characterization of a novel E. coli sessile behaviour termed "crowning" due to the bacterial generation of a new 3-D architectural pattern: a corona. This bacterial pattern is formed by joining bush-like multilayered "coronal flares or spikes" arranged in a ring, which self-organize through the growth, self-clumping and massive self-aggregation of cells tightly interacting inside semisolid agar on plastic surfaces. Remarkably, the corona’s formation is developed independently of the adhesiveness of the major components of E. coli’s EPS matrix, the function of chemotaxis sensory system, type 1 pili and the biofilm master regulator CsgD, but its formation is suppressed by flagella-driven motility and glucose. Intriguingly, this glucose effect on the corona development is not mediated by the classical catabolic repression system, the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Thus, corona formation departs from the canonical regulatory transcriptional core that controls biofilm formation in E. coli. CONCLUSIONS: With this novel "crowning" activity, E. coli expands its repertoire of colonizing collective behaviours to explore, invade and exploit environments whose critical viscosities impede flagella driven-motility. |
format | Online Article Text |
id | pubmed-3936827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39368272014-02-28 Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona Gómez-Gómez, José María Amils, Ricardo BMC Res Notes Research Article BACKGROUND: Encased in a matrix of extracellular polymeric substances (EPS) composed of flagella, adhesins, amyloid fibers (curli), and exopolysaccharides (cellulose, β-1,6-N-acetyl-D-glucosamine polymer-PGA-, colanic acid), the bacteria Escherichia coli is able to attach to and colonize different types of biotic and abiotic surfaces forming biofilms and colonies of intricate morphological architectures. Many of the biological aspects that underlie the generation and development of these E. coli’s formations are largely poorly understood. RESULTS: Here, we report the characterization of a novel E. coli sessile behaviour termed "crowning" due to the bacterial generation of a new 3-D architectural pattern: a corona. This bacterial pattern is formed by joining bush-like multilayered "coronal flares or spikes" arranged in a ring, which self-organize through the growth, self-clumping and massive self-aggregation of cells tightly interacting inside semisolid agar on plastic surfaces. Remarkably, the corona’s formation is developed independently of the adhesiveness of the major components of E. coli’s EPS matrix, the function of chemotaxis sensory system, type 1 pili and the biofilm master regulator CsgD, but its formation is suppressed by flagella-driven motility and glucose. Intriguingly, this glucose effect on the corona development is not mediated by the classical catabolic repression system, the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Thus, corona formation departs from the canonical regulatory transcriptional core that controls biofilm formation in E. coli. CONCLUSIONS: With this novel "crowning" activity, E. coli expands its repertoire of colonizing collective behaviours to explore, invade and exploit environments whose critical viscosities impede flagella driven-motility. BioMed Central 2014-02-25 /pmc/articles/PMC3936827/ /pubmed/24568619 http://dx.doi.org/10.1186/1756-0500-7-108 Text en Copyright © 2014 Gómez-Gómez and Amils; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Gómez-Gómez, José María Amils, Ricardo Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona |
title | Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona |
title_full | Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona |
title_fullStr | Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona |
title_full_unstemmed | Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona |
title_short | Crowning: a novel Escherichia coli colonizing behaviour generating a self-organized corona |
title_sort | crowning: a novel escherichia coli colonizing behaviour generating a self-organized corona |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936827/ https://www.ncbi.nlm.nih.gov/pubmed/24568619 http://dx.doi.org/10.1186/1756-0500-7-108 |
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