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Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland
BACKGROUND: Acinetobacter baumannii is a significant hospital pathogen, possessing a considerable degree of antimicrobial resistance. A. baumannii resistance to carbapenems and aminoglycosides is mostly conferred by class D OXA carbapenemases and aminoglycoside-modifying enzymes, respectively. The a...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936949/ https://www.ncbi.nlm.nih.gov/pubmed/24463791 http://dx.doi.org/10.12659/MSMBR.889811 |
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author | Nowak, Paweł Paluchowska, Paulina Budak, Alicja |
author_facet | Nowak, Paweł Paluchowska, Paulina Budak, Alicja |
author_sort | Nowak, Paweł |
collection | PubMed |
description | BACKGROUND: Acinetobacter baumannii is a significant hospital pathogen, possessing a considerable degree of antimicrobial resistance. A. baumannii resistance to carbapenems and aminoglycosides is mostly conferred by class D OXA carbapenemases and aminoglycoside-modifying enzymes, respectively. The aim of this study was to determine the prevalence of selected genes encoding OXA carbapenemases and aminoglycoside-modifying enzymes in multidrug-resistant strains of A. baumannii. MATERIAL/METHODS: The study included 61 carbapenem-resistant and aminoglycoside-nonsusceptible A. baumannii isolates, collected between 2009 and 2011 in Cracow, Poland. Selected resistance genes, including: bla(OXA-51-like), bla(OXA-23-like), bla(OXA-40-like), bla(OXA-58-like), aac(6′)-Ih, aac(3)-Ia, aac(3)-IIa, aac(6′)-Ib, aph(3′)-Ia and aph(3′)-VI, were detected by PCR method. RESULTS: The bla(OXA-51-like) genes were detected in all isolates, while acquired carbapenemase encoding genes were found in 96.7% of tested strains. Presence of bla(OXA-40-like) and bla(OXA-23-like) genes was observed among 65.6% and 27.9% of isolates, respectively. Assayed aminoglycoside resistance genes were found to harbor 98.4% of isolates. Among tested strains, we observed the following percentages of resistance determinants: aac(3)-Ia – 78.7%, aph(3′)-VI – 78.7% and aph(3′)-Ia – 27.9%. Analysis of co-occurrence of carbapenem and aminoglycoside resistance genes revealed the highest percentage of strains possessing bla(OXA-40-like), aac(3)-Ia, and aph(3′)-VI genes (44.3%). CONCLUSIONS: The bla(OXA-40-like) and aac(3)-Ia/aph(3′)-VI were the most prevalent genes encoding acquired OXA carbapenemases and aminoglycoside-modifying enzymes, respectively, among A. baumannii strains in Cracow, Poland. Genes conferring resistance to carbapenems and aminoglycosides coexisted in the clinical strains of A. baumannii. The phenomenon of A. baumannii resistance indicates the necessity of monitoring for the presence of the resistance genes. |
format | Online Article Text |
id | pubmed-3936949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-39369492014-02-28 Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland Nowak, Paweł Paluchowska, Paulina Budak, Alicja Med Sci Monit Basic Res Laboratory Research BACKGROUND: Acinetobacter baumannii is a significant hospital pathogen, possessing a considerable degree of antimicrobial resistance. A. baumannii resistance to carbapenems and aminoglycosides is mostly conferred by class D OXA carbapenemases and aminoglycoside-modifying enzymes, respectively. The aim of this study was to determine the prevalence of selected genes encoding OXA carbapenemases and aminoglycoside-modifying enzymes in multidrug-resistant strains of A. baumannii. MATERIAL/METHODS: The study included 61 carbapenem-resistant and aminoglycoside-nonsusceptible A. baumannii isolates, collected between 2009 and 2011 in Cracow, Poland. Selected resistance genes, including: bla(OXA-51-like), bla(OXA-23-like), bla(OXA-40-like), bla(OXA-58-like), aac(6′)-Ih, aac(3)-Ia, aac(3)-IIa, aac(6′)-Ib, aph(3′)-Ia and aph(3′)-VI, were detected by PCR method. RESULTS: The bla(OXA-51-like) genes were detected in all isolates, while acquired carbapenemase encoding genes were found in 96.7% of tested strains. Presence of bla(OXA-40-like) and bla(OXA-23-like) genes was observed among 65.6% and 27.9% of isolates, respectively. Assayed aminoglycoside resistance genes were found to harbor 98.4% of isolates. Among tested strains, we observed the following percentages of resistance determinants: aac(3)-Ia – 78.7%, aph(3′)-VI – 78.7% and aph(3′)-Ia – 27.9%. Analysis of co-occurrence of carbapenem and aminoglycoside resistance genes revealed the highest percentage of strains possessing bla(OXA-40-like), aac(3)-Ia, and aph(3′)-VI genes (44.3%). CONCLUSIONS: The bla(OXA-40-like) and aac(3)-Ia/aph(3′)-VI were the most prevalent genes encoding acquired OXA carbapenemases and aminoglycoside-modifying enzymes, respectively, among A. baumannii strains in Cracow, Poland. Genes conferring resistance to carbapenems and aminoglycosides coexisted in the clinical strains of A. baumannii. The phenomenon of A. baumannii resistance indicates the necessity of monitoring for the presence of the resistance genes. International Scientific Literature, Inc. 2014-01-27 /pmc/articles/PMC3936949/ /pubmed/24463791 http://dx.doi.org/10.12659/MSMBR.889811 Text en © Med Sci Monit, 2014 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License |
spellingShingle | Laboratory Research Nowak, Paweł Paluchowska, Paulina Budak, Alicja Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland |
title | Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland |
title_full | Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland |
title_fullStr | Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland |
title_full_unstemmed | Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland |
title_short | Co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of Acinetobacter baumannii from Cracow, Poland |
title_sort | co-occurrence of carbapenem and aminoglycoside resistance genes among multidrug-resistant clinical isolates of acinetobacter baumannii from cracow, poland |
topic | Laboratory Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936949/ https://www.ncbi.nlm.nih.gov/pubmed/24463791 http://dx.doi.org/10.12659/MSMBR.889811 |
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