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Aberrant ADAM10 expression correlates with osteosarcoma progression

BACKGROUND: Osteosarcoma is the most common type of bone cancer and is notorious for its rapid progression. The Notch signaling pathway has recently been shown to be involved in osteosarcoma. As a major sheddase of Notch receptors, ADAM10 has been implicated in many types of cancers, but its role in...

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Autores principales: Zhao, Ren, Ni, Dongjing, Tian, Yi, Ni, Bing, Wang, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936952/
https://www.ncbi.nlm.nih.gov/pubmed/24548763
http://dx.doi.org/10.1186/2047-783X-19-9
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author Zhao, Ren
Ni, Dongjing
Tian, Yi
Ni, Bing
Wang, Aimin
author_facet Zhao, Ren
Ni, Dongjing
Tian, Yi
Ni, Bing
Wang, Aimin
author_sort Zhao, Ren
collection PubMed
description BACKGROUND: Osteosarcoma is the most common type of bone cancer and is notorious for its rapid progression. The Notch signaling pathway has recently been shown to be involved in osteosarcoma. As a major sheddase of Notch receptors, ADAM10 has been implicated in many types of cancers, but its role in osteosarcoma has not been investigated. Previous studies have shown that the expression of CD31 was significantly elevated in metastatic osteosarcoma; however, its expression in nonmetastatic groups is not known. In addition, the mysterious multinucleated giant cell in giant cell-rich osteosarcoma was previously regarded as an osteoclast-like cell, but its exact identity is unclear. METHOD: Tissue chip samples from 40 cases of nonmetastatic osteosarcoma were stained for cytoplasmic ADAM10, activated Notch1 and CD31. Osteoclasts in tumor sections were also stained for tartrate-resistant acid phosphatase (TRAP). RESULTS: Immunofluorescence staining revealed that ADAM10 expression significantly increased with the progression of osteosarcoma as well as in osteoblastic osteosarcoma, whereas the expression of the Notch intracellular domain (NICD) and CD31 was not significantly altered between different pathological stages. In addition, multinucleated giant cells in giant cell-rich osteosarcoma were also found to coexpress CD31, ADAM10 and NICD, but were negative for TRAP staining. CONCLUSIONS: Our results highlight the importance of ADAM10 in the progression of osteosarcoma and suggest that the protein might be a potential therapeutic target in osteosarcoma treatment. This study also demonstrates that the multinucleated giant cell is an angiogenic tumor cell, rather than an osteoclast, and involves ADAM10/Notch1 signaling activation.
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spelling pubmed-39369522014-02-28 Aberrant ADAM10 expression correlates with osteosarcoma progression Zhao, Ren Ni, Dongjing Tian, Yi Ni, Bing Wang, Aimin Eur J Med Res Research BACKGROUND: Osteosarcoma is the most common type of bone cancer and is notorious for its rapid progression. The Notch signaling pathway has recently been shown to be involved in osteosarcoma. As a major sheddase of Notch receptors, ADAM10 has been implicated in many types of cancers, but its role in osteosarcoma has not been investigated. Previous studies have shown that the expression of CD31 was significantly elevated in metastatic osteosarcoma; however, its expression in nonmetastatic groups is not known. In addition, the mysterious multinucleated giant cell in giant cell-rich osteosarcoma was previously regarded as an osteoclast-like cell, but its exact identity is unclear. METHOD: Tissue chip samples from 40 cases of nonmetastatic osteosarcoma were stained for cytoplasmic ADAM10, activated Notch1 and CD31. Osteoclasts in tumor sections were also stained for tartrate-resistant acid phosphatase (TRAP). RESULTS: Immunofluorescence staining revealed that ADAM10 expression significantly increased with the progression of osteosarcoma as well as in osteoblastic osteosarcoma, whereas the expression of the Notch intracellular domain (NICD) and CD31 was not significantly altered between different pathological stages. In addition, multinucleated giant cells in giant cell-rich osteosarcoma were also found to coexpress CD31, ADAM10 and NICD, but were negative for TRAP staining. CONCLUSIONS: Our results highlight the importance of ADAM10 in the progression of osteosarcoma and suggest that the protein might be a potential therapeutic target in osteosarcoma treatment. This study also demonstrates that the multinucleated giant cell is an angiogenic tumor cell, rather than an osteoclast, and involves ADAM10/Notch1 signaling activation. BioMed Central 2014-02-18 /pmc/articles/PMC3936952/ /pubmed/24548763 http://dx.doi.org/10.1186/2047-783X-19-9 Text en Copyright © 2014 Zhao et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhao, Ren
Ni, Dongjing
Tian, Yi
Ni, Bing
Wang, Aimin
Aberrant ADAM10 expression correlates with osteosarcoma progression
title Aberrant ADAM10 expression correlates with osteosarcoma progression
title_full Aberrant ADAM10 expression correlates with osteosarcoma progression
title_fullStr Aberrant ADAM10 expression correlates with osteosarcoma progression
title_full_unstemmed Aberrant ADAM10 expression correlates with osteosarcoma progression
title_short Aberrant ADAM10 expression correlates with osteosarcoma progression
title_sort aberrant adam10 expression correlates with osteosarcoma progression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936952/
https://www.ncbi.nlm.nih.gov/pubmed/24548763
http://dx.doi.org/10.1186/2047-783X-19-9
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