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ILK modulates epithelial polarity and matrix formation in hair follicles

Hair follicle morphogenesis requires coordination of multiple signals and communication between its epithelial and mesenchymal constituents. Cell adhesion protein platforms, which include integrins and integrin-linked kinase (ILK), are critical for hair follicle formation. However, their precise con...

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Detalles Bibliográficos
Autores principales: Rudkouskaya, Alena, Welch, Ian, Dagnino, Lina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937088/
https://www.ncbi.nlm.nih.gov/pubmed/24371086
http://dx.doi.org/10.1091/mbc.E13-08-0499
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author Rudkouskaya, Alena
Welch, Ian
Dagnino, Lina
author_facet Rudkouskaya, Alena
Welch, Ian
Dagnino, Lina
author_sort Rudkouskaya, Alena
collection PubMed
description Hair follicle morphogenesis requires coordination of multiple signals and communication between its epithelial and mesenchymal constituents. Cell adhesion protein platforms, which include integrins and integrin-linked kinase (ILK), are critical for hair follicle formation. However, their precise contribution to this process is poorly understood. We show that in the absence of ILK, the hair follicle matrix lineage fails to develop, likely due to abnormalities in development of apical–basal cell polarity, as well as in laminin-511 and basement membrane assembly at the tip of the hair bud. These defects also result in impaired specification of hair matrix and absence of precortex and inner sheath root cell lineages. The molecular pathways affected in ILK-deficient follicles are similar to those in the absence of epidermal integrin β1 and include Wnt, but not sonic hedgehog, signaling. ILK-deficient hair buds also show abnormalities in the dermal papilla. Addition of exogenous laminin-511 restores morphological and molecular markers associated with hair matrix formation, indicating that ILK regulates hair bud cell polarity and functions upstream from laminin-511 assembly to regulate the developmental progression of hair follicles beyond the germ stage.
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spelling pubmed-39370882014-05-16 ILK modulates epithelial polarity and matrix formation in hair follicles Rudkouskaya, Alena Welch, Ian Dagnino, Lina Mol Biol Cell Articles Hair follicle morphogenesis requires coordination of multiple signals and communication between its epithelial and mesenchymal constituents. Cell adhesion protein platforms, which include integrins and integrin-linked kinase (ILK), are critical for hair follicle formation. However, their precise contribution to this process is poorly understood. We show that in the absence of ILK, the hair follicle matrix lineage fails to develop, likely due to abnormalities in development of apical–basal cell polarity, as well as in laminin-511 and basement membrane assembly at the tip of the hair bud. These defects also result in impaired specification of hair matrix and absence of precortex and inner sheath root cell lineages. The molecular pathways affected in ILK-deficient follicles are similar to those in the absence of epidermal integrin β1 and include Wnt, but not sonic hedgehog, signaling. ILK-deficient hair buds also show abnormalities in the dermal papilla. Addition of exogenous laminin-511 restores morphological and molecular markers associated with hair matrix formation, indicating that ILK regulates hair bud cell polarity and functions upstream from laminin-511 assembly to regulate the developmental progression of hair follicles beyond the germ stage. The American Society for Cell Biology 2014-03-01 /pmc/articles/PMC3937088/ /pubmed/24371086 http://dx.doi.org/10.1091/mbc.E13-08-0499 Text en © 2014 Rudkouskaya et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Rudkouskaya, Alena
Welch, Ian
Dagnino, Lina
ILK modulates epithelial polarity and matrix formation in hair follicles
title ILK modulates epithelial polarity and matrix formation in hair follicles
title_full ILK modulates epithelial polarity and matrix formation in hair follicles
title_fullStr ILK modulates epithelial polarity and matrix formation in hair follicles
title_full_unstemmed ILK modulates epithelial polarity and matrix formation in hair follicles
title_short ILK modulates epithelial polarity and matrix formation in hair follicles
title_sort ilk modulates epithelial polarity and matrix formation in hair follicles
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937088/
https://www.ncbi.nlm.nih.gov/pubmed/24371086
http://dx.doi.org/10.1091/mbc.E13-08-0499
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