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Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation

BACKGROUND: In neuroinflammatory diseases, macrophages can play a dual role in the process of tissue damage, depending on their activation status (M1 / M2). M1 macrophages are considered to exert damaging effects to neurons, whereas M2 macrophages are reported to aid regeneration and repair of neuro...

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Autores principales: Vogel, Daphne YS, Heijnen, Priscilla DAM, Breur, Marjolein, de Vries, Helga E, Tool, Anton TJ, Amor, Sandra, Dijkstra, Christine D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937114/
https://www.ncbi.nlm.nih.gov/pubmed/24485070
http://dx.doi.org/10.1186/1742-2094-11-23
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author Vogel, Daphne YS
Heijnen, Priscilla DAM
Breur, Marjolein
de Vries, Helga E
Tool, Anton TJ
Amor, Sandra
Dijkstra, Christine D
author_facet Vogel, Daphne YS
Heijnen, Priscilla DAM
Breur, Marjolein
de Vries, Helga E
Tool, Anton TJ
Amor, Sandra
Dijkstra, Christine D
author_sort Vogel, Daphne YS
collection PubMed
description BACKGROUND: In neuroinflammatory diseases, macrophages can play a dual role in the process of tissue damage, depending on their activation status (M1 / M2). M1 macrophages are considered to exert damaging effects to neurons, whereas M2 macrophages are reported to aid regeneration and repair of neurons. Their migration within the central nervous system may be of critical importance in the final outcome of neurodegeneration in neuroinflammatory diseases e.g. multiple sclerosis (MS). To provide insight into this process, we examined the migratory capacity of human monocyte-derived M1 and M2 polarised macrophages towards chemoattractants, relevant for neuroinflammatory diseases like MS. METHODS: Primary cultures of human monocyte-derived macrophages were exposed to interferon gamma and lipopolysaccharide (LPS) to evoke proinflammatory (M1) activation or IL-4 to evoke anti-inflammatory (M2) activation. In a TAXIScan assay, migration of M0, M1 and M2 towards chemoattractants was measured and quantified. Furthermore the adhesion capacity and the expression levels of integrins as well as chemokine receptors of M0, M1 and M2 were assessed. Alterations in cell morphology were analysed using fluorescent labelling of the cytoskeleton. RESULTS: Significant differences were observed between M1 and M2 macrophages in the migration towards chemoattractants. We show that M2 macrophages migrated over longer distances towards CCL2, CCL5, CXCL10, CXCL12 and C1q compared to non-activated (M0) and M1 macrophages. No differences were observed in the adhesion of M0, M1 and M2 macrophages to multiple matrix components, nor in the expression of integrins and chemokine receptors. Significant changes were observed in the cytoskeleton organization upon stimulation with CCL2, M0, M1 and M2 macrophages adopt a spherical morphology and the cytoskeleton is rapidly rearranged. M0 and M2 macrophages are able to form filopodia, whereas M1 macrophages only adapt a spherical morphology. CONCLUSIONS: Together our results indicate that the alternative activation status of macrophages promotes their migratory properties to chemoattractants relevant for neuroinflammatory diseases like MS. Conversely, classically activated, proinflammatory macrophages have reduced migratory properties. Based on our results, we postulate that the activation status of the macrophage influences the capacity of the macrophages to rearrange their cytoskeleton. This is the first step in understanding how modulation of macrophage activation affects macrophage migration in neuroinflammatory diseases like MS.
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spelling pubmed-39371142014-02-28 Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation Vogel, Daphne YS Heijnen, Priscilla DAM Breur, Marjolein de Vries, Helga E Tool, Anton TJ Amor, Sandra Dijkstra, Christine D J Neuroinflammation Research BACKGROUND: In neuroinflammatory diseases, macrophages can play a dual role in the process of tissue damage, depending on their activation status (M1 / M2). M1 macrophages are considered to exert damaging effects to neurons, whereas M2 macrophages are reported to aid regeneration and repair of neurons. Their migration within the central nervous system may be of critical importance in the final outcome of neurodegeneration in neuroinflammatory diseases e.g. multiple sclerosis (MS). To provide insight into this process, we examined the migratory capacity of human monocyte-derived M1 and M2 polarised macrophages towards chemoattractants, relevant for neuroinflammatory diseases like MS. METHODS: Primary cultures of human monocyte-derived macrophages were exposed to interferon gamma and lipopolysaccharide (LPS) to evoke proinflammatory (M1) activation or IL-4 to evoke anti-inflammatory (M2) activation. In a TAXIScan assay, migration of M0, M1 and M2 towards chemoattractants was measured and quantified. Furthermore the adhesion capacity and the expression levels of integrins as well as chemokine receptors of M0, M1 and M2 were assessed. Alterations in cell morphology were analysed using fluorescent labelling of the cytoskeleton. RESULTS: Significant differences were observed between M1 and M2 macrophages in the migration towards chemoattractants. We show that M2 macrophages migrated over longer distances towards CCL2, CCL5, CXCL10, CXCL12 and C1q compared to non-activated (M0) and M1 macrophages. No differences were observed in the adhesion of M0, M1 and M2 macrophages to multiple matrix components, nor in the expression of integrins and chemokine receptors. Significant changes were observed in the cytoskeleton organization upon stimulation with CCL2, M0, M1 and M2 macrophages adopt a spherical morphology and the cytoskeleton is rapidly rearranged. M0 and M2 macrophages are able to form filopodia, whereas M1 macrophages only adapt a spherical morphology. CONCLUSIONS: Together our results indicate that the alternative activation status of macrophages promotes their migratory properties to chemoattractants relevant for neuroinflammatory diseases like MS. Conversely, classically activated, proinflammatory macrophages have reduced migratory properties. Based on our results, we postulate that the activation status of the macrophage influences the capacity of the macrophages to rearrange their cytoskeleton. This is the first step in understanding how modulation of macrophage activation affects macrophage migration in neuroinflammatory diseases like MS. BioMed Central 2014-02-01 /pmc/articles/PMC3937114/ /pubmed/24485070 http://dx.doi.org/10.1186/1742-2094-11-23 Text en Copyright © 2014 Vogel et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Vogel, Daphne YS
Heijnen, Priscilla DAM
Breur, Marjolein
de Vries, Helga E
Tool, Anton TJ
Amor, Sandra
Dijkstra, Christine D
Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
title Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
title_full Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
title_fullStr Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
title_full_unstemmed Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
title_short Macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
title_sort macrophages migrate in an activation-dependent manner to chemokines involved in neuroinflammation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937114/
https://www.ncbi.nlm.nih.gov/pubmed/24485070
http://dx.doi.org/10.1186/1742-2094-11-23
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