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Immunohistochemical expression of MMP-14 and MMP-2, and MMP-2 activity during human ovarian follicular development

BACKGROUND: The aim of this study was to investigate the presence of MMP-14 and MMP-2 during human ovarian follicular development using immunohistochemistry, and the activity of MMP-2 in follicular fluid using zymography. METHODS: Ovarian tissue collected from the archives of the Department of Patho...

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Detalles Bibliográficos
Autores principales: Vos, Maria Caroline, van der Wurff, Anneke AM, Last, Jessie TJ, de Boed, Ella AM, Smeenk, Jesper MJ, van Kuppevelt, Toin H, Massuger, Leon FAG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937151/
https://www.ncbi.nlm.nih.gov/pubmed/24485069
http://dx.doi.org/10.1186/1477-7827-12-12
Descripción
Sumario:BACKGROUND: The aim of this study was to investigate the presence of MMP-14 and MMP-2 during human ovarian follicular development using immunohistochemistry, and the activity of MMP-2 in follicular fluid using zymography. METHODS: Ovarian tissue collected from the archives of the Department of Pathology was examined and medical records and histopathology were reviewed. Follicular fluids were collected at the IVF-department and analyzed using zymography. RESULTS: MMP-14 and MMP-2 were increasingly found in the growing follicles and MMP-2 was highly expressed in the corpus luteum. Pro-MMP-2 was present in follicular fluid of IVF-patients. CONCLUSIONS: The presence of MMP-14 and MMP-2 during human ovarian follicular development from the primordial follicle to the tertiary follicle and corpus luteum is confirmed, as was indicated by earlier animal studies following stimulation with gonadotrophins.