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Dramatic reduction of culture time of Mycobacterium tuberculosis
Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shorten...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937792/ https://www.ncbi.nlm.nih.gov/pubmed/24577292 http://dx.doi.org/10.1038/srep04236 |
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author | Ghodbane, Ramzi Raoult, Didier Drancourt, Michel |
author_facet | Ghodbane, Ramzi Raoult, Didier Drancourt, Michel |
author_sort | Ghodbane, Ramzi |
collection | PubMed |
description | Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shortened this delay. In the best case, primary culture and rifampicin susceptibility testing were achieved in 72 hours when specimens were inoculated directly on the medium supplemented by antibiotic at the beginning of the culture. |
format | Online Article Text |
id | pubmed-3937792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-39377922014-03-04 Dramatic reduction of culture time of Mycobacterium tuberculosis Ghodbane, Ramzi Raoult, Didier Drancourt, Michel Sci Rep Article Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shortened this delay. In the best case, primary culture and rifampicin susceptibility testing were achieved in 72 hours when specimens were inoculated directly on the medium supplemented by antibiotic at the beginning of the culture. Nature Publishing Group 2014-02-28 /pmc/articles/PMC3937792/ /pubmed/24577292 http://dx.doi.org/10.1038/srep04236 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Ghodbane, Ramzi Raoult, Didier Drancourt, Michel Dramatic reduction of culture time of Mycobacterium tuberculosis |
title | Dramatic reduction of culture time of Mycobacterium tuberculosis |
title_full | Dramatic reduction of culture time of Mycobacterium tuberculosis |
title_fullStr | Dramatic reduction of culture time of Mycobacterium tuberculosis |
title_full_unstemmed | Dramatic reduction of culture time of Mycobacterium tuberculosis |
title_short | Dramatic reduction of culture time of Mycobacterium tuberculosis |
title_sort | dramatic reduction of culture time of mycobacterium tuberculosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937792/ https://www.ncbi.nlm.nih.gov/pubmed/24577292 http://dx.doi.org/10.1038/srep04236 |
work_keys_str_mv | AT ghodbaneramzi dramaticreductionofculturetimeofmycobacteriumtuberculosis AT raoultdidier dramaticreductionofculturetimeofmycobacteriumtuberculosis AT drancourtmichel dramaticreductionofculturetimeofmycobacteriumtuberculosis |