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Dramatic reduction of culture time of Mycobacterium tuberculosis

Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shorten...

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Detalles Bibliográficos
Autores principales: Ghodbane, Ramzi, Raoult, Didier, Drancourt, Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937792/
https://www.ncbi.nlm.nih.gov/pubmed/24577292
http://dx.doi.org/10.1038/srep04236
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author Ghodbane, Ramzi
Raoult, Didier
Drancourt, Michel
author_facet Ghodbane, Ramzi
Raoult, Didier
Drancourt, Michel
author_sort Ghodbane, Ramzi
collection PubMed
description Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shortened this delay. In the best case, primary culture and rifampicin susceptibility testing were achieved in 72 hours when specimens were inoculated directly on the medium supplemented by antibiotic at the beginning of the culture.
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spelling pubmed-39377922014-03-04 Dramatic reduction of culture time of Mycobacterium tuberculosis Ghodbane, Ramzi Raoult, Didier Drancourt, Michel Sci Rep Article Mycobacterium tuberculosis culture, a critical technique for routine diagnosis of tuberculosis, takes more than two weeks. Here, step-by-step improvements in the protocol including a new medium, microaerophlic atmosphere or ascorbic-acid supplement and autofluorescence detection dramatically shortened this delay. In the best case, primary culture and rifampicin susceptibility testing were achieved in 72 hours when specimens were inoculated directly on the medium supplemented by antibiotic at the beginning of the culture. Nature Publishing Group 2014-02-28 /pmc/articles/PMC3937792/ /pubmed/24577292 http://dx.doi.org/10.1038/srep04236 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Ghodbane, Ramzi
Raoult, Didier
Drancourt, Michel
Dramatic reduction of culture time of Mycobacterium tuberculosis
title Dramatic reduction of culture time of Mycobacterium tuberculosis
title_full Dramatic reduction of culture time of Mycobacterium tuberculosis
title_fullStr Dramatic reduction of culture time of Mycobacterium tuberculosis
title_full_unstemmed Dramatic reduction of culture time of Mycobacterium tuberculosis
title_short Dramatic reduction of culture time of Mycobacterium tuberculosis
title_sort dramatic reduction of culture time of mycobacterium tuberculosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937792/
https://www.ncbi.nlm.nih.gov/pubmed/24577292
http://dx.doi.org/10.1038/srep04236
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