Cargando…

Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB...

Descripción completa

Detalles Bibliográficos
Autores principales: Abadi, Amin Talebi Bezmin, Mobarez, Ashraf Mohhabati, Bonten, Marc JM, Wagenaar, Jaap A, Kusters, Johannes G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938475/
https://www.ncbi.nlm.nih.gov/pubmed/24552154
http://dx.doi.org/10.1186/1471-230X-14-33
_version_ 1782305607316930560
author Abadi, Amin Talebi Bezmin
Mobarez, Ashraf Mohhabati
Bonten, Marc JM
Wagenaar, Jaap A
Kusters, Johannes G
author_facet Abadi, Amin Talebi Bezmin
Mobarez, Ashraf Mohhabati
Bonten, Marc JM
Wagenaar, Jaap A
Kusters, Johannes G
author_sort Abadi, Amin Talebi Bezmin
collection PubMed
description BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases. METHODS: A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients. RESULTS: Of 360H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17–73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group. CONCLUSIONS: The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer.
format Online
Article
Text
id pubmed-3938475
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-39384752014-03-01 Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori Abadi, Amin Talebi Bezmin Mobarez, Ashraf Mohhabati Bonten, Marc JM Wagenaar, Jaap A Kusters, Johannes G BMC Gastroenterol Research Article BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases. METHODS: A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients. RESULTS: Of 360H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17–73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group. CONCLUSIONS: The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer. BioMed Central 2014-02-19 /pmc/articles/PMC3938475/ /pubmed/24552154 http://dx.doi.org/10.1186/1471-230X-14-33 Text en Copyright © 2014 Abadi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research Article
Abadi, Amin Talebi Bezmin
Mobarez, Ashraf Mohhabati
Bonten, Marc JM
Wagenaar, Jaap A
Kusters, Johannes G
Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori
title Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori
title_full Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori
title_fullStr Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori
title_full_unstemmed Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori
title_short Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori
title_sort clinical relevance of the caga, tnpa and tnpb genes in helicobacter pylori
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938475/
https://www.ncbi.nlm.nih.gov/pubmed/24552154
http://dx.doi.org/10.1186/1471-230X-14-33
work_keys_str_mv AT abadiamintalebibezmin clinicalrelevanceofthecagatnpaandtnpbgenesinhelicobacterpylori
AT mobarezashrafmohhabati clinicalrelevanceofthecagatnpaandtnpbgenesinhelicobacterpylori
AT bontenmarcjm clinicalrelevanceofthecagatnpaandtnpbgenesinhelicobacterpylori
AT wagenaarjaapa clinicalrelevanceofthecagatnpaandtnpbgenesinhelicobacterpylori
AT kustersjohannesg clinicalrelevanceofthecagatnpaandtnpbgenesinhelicobacterpylori