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Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway
Baicalein, one of the major flavonids in Scutellaria baicalensis, has historically been used in anti-inflammatory and anti-cancer therapies. However, the anti-metastatic effect and related mechanism(s) in glioma are still unclear. In this study, we thus utilized glioma cell lines U87MG and U251MG to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938668/ https://www.ncbi.nlm.nih.gov/pubmed/24587321 http://dx.doi.org/10.1371/journal.pone.0090318 |
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author | Zhang, Zhenni Lv, Jianrui Lei, Xiaoming Li, Siyuan Zhang, Yong Meng, Lihua Xue, Rongliang Li, Zongfang |
author_facet | Zhang, Zhenni Lv, Jianrui Lei, Xiaoming Li, Siyuan Zhang, Yong Meng, Lihua Xue, Rongliang Li, Zongfang |
author_sort | Zhang, Zhenni |
collection | PubMed |
description | Baicalein, one of the major flavonids in Scutellaria baicalensis, has historically been used in anti-inflammatory and anti-cancer therapies. However, the anti-metastatic effect and related mechanism(s) in glioma are still unclear. In this study, we thus utilized glioma cell lines U87MG and U251MG to explore the effect of baicalein. We found that administration of baicalein significantly inhibited migration and invasion of glioma cells. In addition, after treating with baicalein for 24 h, there was a decrease in the levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 expression as well as proteinase activity in glioma cells. Conversely, the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 was increased in a dose-dependent manner. Moreover, baicalein treatment significantly decreased the phosphorylated level of p38, but not ERK1/2, JNK1/2 and PI3K/Akt. Combined treatment with a p38 inhibitor (SB203580) and baicalein resulted in the synergistic reduction of MMP-2 and MMP-9 expression and then increase of TIMP-1 and TIMP-2 expression; and the invasive capabilities of U87MG cells were also inhibited. However, p38 chemical activator (anisomycin) could block these effects produced by baicalein, suggesting baicalein directly downregulate the p38 signaling pathway. In conclusion, baicalein inhibits glioma cells invasion and metastasis by reducing cell motility and migration via suppression of p38 signaling pathway, suggesting that baicalein is a potential therapeutic agent for glioma. |
format | Online Article Text |
id | pubmed-3938668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39386682014-03-04 Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway Zhang, Zhenni Lv, Jianrui Lei, Xiaoming Li, Siyuan Zhang, Yong Meng, Lihua Xue, Rongliang Li, Zongfang PLoS One Research Article Baicalein, one of the major flavonids in Scutellaria baicalensis, has historically been used in anti-inflammatory and anti-cancer therapies. However, the anti-metastatic effect and related mechanism(s) in glioma are still unclear. In this study, we thus utilized glioma cell lines U87MG and U251MG to explore the effect of baicalein. We found that administration of baicalein significantly inhibited migration and invasion of glioma cells. In addition, after treating with baicalein for 24 h, there was a decrease in the levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 expression as well as proteinase activity in glioma cells. Conversely, the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 was increased in a dose-dependent manner. Moreover, baicalein treatment significantly decreased the phosphorylated level of p38, but not ERK1/2, JNK1/2 and PI3K/Akt. Combined treatment with a p38 inhibitor (SB203580) and baicalein resulted in the synergistic reduction of MMP-2 and MMP-9 expression and then increase of TIMP-1 and TIMP-2 expression; and the invasive capabilities of U87MG cells were also inhibited. However, p38 chemical activator (anisomycin) could block these effects produced by baicalein, suggesting baicalein directly downregulate the p38 signaling pathway. In conclusion, baicalein inhibits glioma cells invasion and metastasis by reducing cell motility and migration via suppression of p38 signaling pathway, suggesting that baicalein is a potential therapeutic agent for glioma. Public Library of Science 2014-02-28 /pmc/articles/PMC3938668/ /pubmed/24587321 http://dx.doi.org/10.1371/journal.pone.0090318 Text en © 2014 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Zhenni Lv, Jianrui Lei, Xiaoming Li, Siyuan Zhang, Yong Meng, Lihua Xue, Rongliang Li, Zongfang Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway |
title | Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway |
title_full | Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway |
title_fullStr | Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway |
title_full_unstemmed | Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway |
title_short | Baicalein Reduces the Invasion of Glioma Cells via Reducing the Activity of p38 Signaling Pathway |
title_sort | baicalein reduces the invasion of glioma cells via reducing the activity of p38 signaling pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938668/ https://www.ncbi.nlm.nih.gov/pubmed/24587321 http://dx.doi.org/10.1371/journal.pone.0090318 |
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