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Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India
Previously we reported that the exposure to hepatitis B virus (HBV) infection serves as a major threat among the treatment naive HIV infected population of eastern India. Hence, molecular characterization of these strains is of utmost importance in order to identify clinically significant HBV mutati...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938687/ https://www.ncbi.nlm.nih.gov/pubmed/24587360 http://dx.doi.org/10.1371/journal.pone.0090432 |
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author | Saha, Debraj Pal, Ananya Biswas, Avik Panigrahi, Rajesh Sarkar, Neelakshi Das, Dipanwita Sarkar, Jayeeta Guha, Subhasish Kamal Saha, Bibhuti Chakrabarti, Sekhar Chakravarty, Runu |
author_facet | Saha, Debraj Pal, Ananya Biswas, Avik Panigrahi, Rajesh Sarkar, Neelakshi Das, Dipanwita Sarkar, Jayeeta Guha, Subhasish Kamal Saha, Bibhuti Chakrabarti, Sekhar Chakravarty, Runu |
author_sort | Saha, Debraj |
collection | PubMed |
description | Previously we reported that the exposure to hepatitis B virus (HBV) infection serves as a major threat among the treatment naive HIV infected population of eastern India. Hence, molecular characterization of these strains is of utmost importance in order to identify clinically significant HBV mutations. A total of 85 treatment naive HIV/HBV co-infected participants were included of whom the complete basal core promoter/precore region, the core and the whole envelope gene could be successfully sequenced for 59, 57 and 39 isolates respectively. Following phylogenetic analysis, it was found that HBV/D was the predominant genotype with HBV/D2 (38.5%) being the most prevalent subgenotype followed by HBV/A1. The major mutations affecting HBeAg expression includes the A1762T/G1764A (13.6%), G1896A (22%) and G1862T mutation (33.9%) which was predominantly associated with HBV/A1. Moreover, the prevalence of G1896A was considerably high among the HBeAg negative HIV/HBV co-infected subjects compared to HBV mono-infection. The main amino acid substitutions within the MHC class II restricted T-cell epitope of HBcAg includes the T12S (15.8%) and T67N (12.3%) mutation and the V27I (10.5%) mutation in the MHC class I restricted T-cell epitope. PreS1/S2 deletion was detected in 3 isolates with all harboring the BCP double mutation. Furthermore, the frequently occurring mutations in the major hydrophilic loop of the S gene include the T125M, A128V and M133I/L. Therefore, this study is the first from India to report useful information on the molecular heterogeneity of the HBV strains circulating among the treatment naive HIV/HBV co-infected population and is thus clinically relevant. |
format | Online Article Text |
id | pubmed-3938687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39386872014-03-04 Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India Saha, Debraj Pal, Ananya Biswas, Avik Panigrahi, Rajesh Sarkar, Neelakshi Das, Dipanwita Sarkar, Jayeeta Guha, Subhasish Kamal Saha, Bibhuti Chakrabarti, Sekhar Chakravarty, Runu PLoS One Research Article Previously we reported that the exposure to hepatitis B virus (HBV) infection serves as a major threat among the treatment naive HIV infected population of eastern India. Hence, molecular characterization of these strains is of utmost importance in order to identify clinically significant HBV mutations. A total of 85 treatment naive HIV/HBV co-infected participants were included of whom the complete basal core promoter/precore region, the core and the whole envelope gene could be successfully sequenced for 59, 57 and 39 isolates respectively. Following phylogenetic analysis, it was found that HBV/D was the predominant genotype with HBV/D2 (38.5%) being the most prevalent subgenotype followed by HBV/A1. The major mutations affecting HBeAg expression includes the A1762T/G1764A (13.6%), G1896A (22%) and G1862T mutation (33.9%) which was predominantly associated with HBV/A1. Moreover, the prevalence of G1896A was considerably high among the HBeAg negative HIV/HBV co-infected subjects compared to HBV mono-infection. The main amino acid substitutions within the MHC class II restricted T-cell epitope of HBcAg includes the T12S (15.8%) and T67N (12.3%) mutation and the V27I (10.5%) mutation in the MHC class I restricted T-cell epitope. PreS1/S2 deletion was detected in 3 isolates with all harboring the BCP double mutation. Furthermore, the frequently occurring mutations in the major hydrophilic loop of the S gene include the T125M, A128V and M133I/L. Therefore, this study is the first from India to report useful information on the molecular heterogeneity of the HBV strains circulating among the treatment naive HIV/HBV co-infected population and is thus clinically relevant. Public Library of Science 2014-02-28 /pmc/articles/PMC3938687/ /pubmed/24587360 http://dx.doi.org/10.1371/journal.pone.0090432 Text en © 2014 Saha et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Saha, Debraj Pal, Ananya Biswas, Avik Panigrahi, Rajesh Sarkar, Neelakshi Das, Dipanwita Sarkar, Jayeeta Guha, Subhasish Kamal Saha, Bibhuti Chakrabarti, Sekhar Chakravarty, Runu Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India |
title | Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India |
title_full | Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India |
title_fullStr | Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India |
title_full_unstemmed | Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India |
title_short | Molecular Characterization of HBV Strains Circulating among the Treatment-Naive HIV/HBV Co-Infected Patients of Eastern India |
title_sort | molecular characterization of hbv strains circulating among the treatment-naive hiv/hbv co-infected patients of eastern india |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938687/ https://www.ncbi.nlm.nih.gov/pubmed/24587360 http://dx.doi.org/10.1371/journal.pone.0090432 |
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