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Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis
Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938718/ https://www.ncbi.nlm.nih.gov/pubmed/24587351 http://dx.doi.org/10.1371/journal.pone.0090398 |
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author | Bareja, Akshay Holt, Jason A. Luo, Guizhen Chang, Calvin Lin, Junyu Hinken, Aaron C. Freudenberg, Johannes M. Kraus, William E. Evans, William J. Billin, Andrew N. |
author_facet | Bareja, Akshay Holt, Jason A. Luo, Guizhen Chang, Calvin Lin, Junyu Hinken, Aaron C. Freudenberg, Johannes M. Kraus, William E. Evans, William J. Billin, Andrew N. |
author_sort | Bareja, Akshay |
collection | PubMed |
description | Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease. |
format | Online Article Text |
id | pubmed-3938718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39387182014-03-04 Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis Bareja, Akshay Holt, Jason A. Luo, Guizhen Chang, Calvin Lin, Junyu Hinken, Aaron C. Freudenberg, Johannes M. Kraus, William E. Evans, William J. Billin, Andrew N. PLoS One Research Article Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease. Public Library of Science 2014-02-28 /pmc/articles/PMC3938718/ /pubmed/24587351 http://dx.doi.org/10.1371/journal.pone.0090398 Text en © 2014 Bareja et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bareja, Akshay Holt, Jason A. Luo, Guizhen Chang, Calvin Lin, Junyu Hinken, Aaron C. Freudenberg, Johannes M. Kraus, William E. Evans, William J. Billin, Andrew N. Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis |
title | Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis |
title_full | Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis |
title_fullStr | Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis |
title_full_unstemmed | Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis |
title_short | Human and Mouse Skeletal Muscle Stem Cells: Convergent and Divergent Mechanisms of Myogenesis |
title_sort | human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3938718/ https://www.ncbi.nlm.nih.gov/pubmed/24587351 http://dx.doi.org/10.1371/journal.pone.0090398 |
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