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An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration

A transcriptional programme initiated by the proneural factors Neurog2 and Ascl1 controls successive steps of neurogenesis in the embryonic cerebral cortex. Previous work has shown that proneural factors also confer a migratory behaviour to cortical neurons by inducing the expression of the small GT...

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Autores principales: Azzarelli, Roberta, Pacary, Emilie, Garg, Ritu, Garcez, Patricia, van den Berg, Debbie, Riou, Philippe, Ridley, Anne J., Friedel, Roland H., Parsons, Maddy, Guillemot, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939360/
https://www.ncbi.nlm.nih.gov/pubmed/24572910
http://dx.doi.org/10.1038/ncomms4405
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author Azzarelli, Roberta
Pacary, Emilie
Garg, Ritu
Garcez, Patricia
van den Berg, Debbie
Riou, Philippe
Ridley, Anne J.
Friedel, Roland H.
Parsons, Maddy
Guillemot, François
author_facet Azzarelli, Roberta
Pacary, Emilie
Garg, Ritu
Garcez, Patricia
van den Berg, Debbie
Riou, Philippe
Ridley, Anne J.
Friedel, Roland H.
Parsons, Maddy
Guillemot, François
author_sort Azzarelli, Roberta
collection PubMed
description A transcriptional programme initiated by the proneural factors Neurog2 and Ascl1 controls successive steps of neurogenesis in the embryonic cerebral cortex. Previous work has shown that proneural factors also confer a migratory behaviour to cortical neurons by inducing the expression of the small GTP-binding proteins such as Rnd2 and Rnd3. However, the directionality of radial migration suggests that migrating neurons also respond to extracellular signal-regulated pathways. Here we show that the Plexin B2 receptor interacts physically and functionally with Rnd3 and stimulates RhoA activity in migrating cortical neurons. Plexin B2 competes with p190RhoGAP for binding to Rnd3, thus blocking the Rnd3-mediated inhibition of RhoA and also recruits RhoGEFs to directly stimulate RhoA activity. Thus, an interaction between the cell-extrinsic Plexin signalling pathway and the cell-intrinsic Ascl1-Rnd3 pathway determines the level of RhoA activity appropriate for cortical neuron migration.
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spelling pubmed-39393602014-03-10 An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration Azzarelli, Roberta Pacary, Emilie Garg, Ritu Garcez, Patricia van den Berg, Debbie Riou, Philippe Ridley, Anne J. Friedel, Roland H. Parsons, Maddy Guillemot, François Nat Commun Article A transcriptional programme initiated by the proneural factors Neurog2 and Ascl1 controls successive steps of neurogenesis in the embryonic cerebral cortex. Previous work has shown that proneural factors also confer a migratory behaviour to cortical neurons by inducing the expression of the small GTP-binding proteins such as Rnd2 and Rnd3. However, the directionality of radial migration suggests that migrating neurons also respond to extracellular signal-regulated pathways. Here we show that the Plexin B2 receptor interacts physically and functionally with Rnd3 and stimulates RhoA activity in migrating cortical neurons. Plexin B2 competes with p190RhoGAP for binding to Rnd3, thus blocking the Rnd3-mediated inhibition of RhoA and also recruits RhoGEFs to directly stimulate RhoA activity. Thus, an interaction between the cell-extrinsic Plexin signalling pathway and the cell-intrinsic Ascl1-Rnd3 pathway determines the level of RhoA activity appropriate for cortical neuron migration. Nature Pub. Group 2014-02-27 /pmc/articles/PMC3939360/ /pubmed/24572910 http://dx.doi.org/10.1038/ncomms4405 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Article
Azzarelli, Roberta
Pacary, Emilie
Garg, Ritu
Garcez, Patricia
van den Berg, Debbie
Riou, Philippe
Ridley, Anne J.
Friedel, Roland H.
Parsons, Maddy
Guillemot, François
An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration
title An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration
title_full An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration
title_fullStr An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration
title_full_unstemmed An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration
title_short An antagonistic interaction between PlexinB2 and Rnd3 controls RhoA activity and cortical neuron migration
title_sort antagonistic interaction between plexinb2 and rnd3 controls rhoa activity and cortical neuron migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939360/
https://www.ncbi.nlm.nih.gov/pubmed/24572910
http://dx.doi.org/10.1038/ncomms4405
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