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Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain

Bacteria associated with mammals are a rich source of microbial biodiversity; however, little is known concerning the abilities of these microbes to generate secondary metabolites. This report focuses on a bacterium isolated from the ear of a feral hog from southwestern Oklahoma, USA. The bacterium...

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Autores principales: Theodore, Christine M., Stamps, Blake W., King, Jarrod B., Price, Lauren S. L., Powell, Douglas R., Stevenson, Bradley S., Cichewicz, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940840/
https://www.ncbi.nlm.nih.gov/pubmed/24595070
http://dx.doi.org/10.1371/journal.pone.0090124
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author Theodore, Christine M.
Stamps, Blake W.
King, Jarrod B.
Price, Lauren S. L.
Powell, Douglas R.
Stevenson, Bradley S.
Cichewicz, Robert H.
author_facet Theodore, Christine M.
Stamps, Blake W.
King, Jarrod B.
Price, Lauren S. L.
Powell, Douglas R.
Stevenson, Bradley S.
Cichewicz, Robert H.
author_sort Theodore, Christine M.
collection PubMed
description Bacteria associated with mammals are a rich source of microbial biodiversity; however, little is known concerning the abilities of these microbes to generate secondary metabolites. This report focuses on a bacterium isolated from the ear of a feral hog from southwestern Oklahoma, USA. The bacterium was identified as a new strain (PE36) of Brevibacillus latersporus, which was shown via genomic analysis to contain a large number of gene clusters presumably involved in secondary metabolite biosynthesis. A scale-up culture of B. latersporus PE36 yielded three bioactive compounds that inhibited the growth of methicillin-resistant Staphylococcus aureus (basiliskamides A and B and 12-methyltetradecanoic acid). Further studies of the isolate's secondary metabolome provided both new (auripyrazine) and previously-described pyrazine-containing compounds. In addition, a new peptidic natural product (auriporcine) was purified that was determined to be composed of a polyketide unit, two L-proline residues, two D-leucine residues, one L-leucine residue, and a reduced L-phenylalanine (L-phenylalanol). An examination of the genome revealed two gene clusters that are likely responsible for generating the basiliskamides and auriporcine. These combined genomic and chemical studies confirm that new and unusual secondary metabolites can be obtained from the bacterial associates of wild mammals.
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spelling pubmed-39408402014-03-06 Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain Theodore, Christine M. Stamps, Blake W. King, Jarrod B. Price, Lauren S. L. Powell, Douglas R. Stevenson, Bradley S. Cichewicz, Robert H. PLoS One Research Article Bacteria associated with mammals are a rich source of microbial biodiversity; however, little is known concerning the abilities of these microbes to generate secondary metabolites. This report focuses on a bacterium isolated from the ear of a feral hog from southwestern Oklahoma, USA. The bacterium was identified as a new strain (PE36) of Brevibacillus latersporus, which was shown via genomic analysis to contain a large number of gene clusters presumably involved in secondary metabolite biosynthesis. A scale-up culture of B. latersporus PE36 yielded three bioactive compounds that inhibited the growth of methicillin-resistant Staphylococcus aureus (basiliskamides A and B and 12-methyltetradecanoic acid). Further studies of the isolate's secondary metabolome provided both new (auripyrazine) and previously-described pyrazine-containing compounds. In addition, a new peptidic natural product (auriporcine) was purified that was determined to be composed of a polyketide unit, two L-proline residues, two D-leucine residues, one L-leucine residue, and a reduced L-phenylalanine (L-phenylalanol). An examination of the genome revealed two gene clusters that are likely responsible for generating the basiliskamides and auriporcine. These combined genomic and chemical studies confirm that new and unusual secondary metabolites can be obtained from the bacterial associates of wild mammals. Public Library of Science 2014-03-03 /pmc/articles/PMC3940840/ /pubmed/24595070 http://dx.doi.org/10.1371/journal.pone.0090124 Text en © 2014 Theodore et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Theodore, Christine M.
Stamps, Blake W.
King, Jarrod B.
Price, Lauren S. L.
Powell, Douglas R.
Stevenson, Bradley S.
Cichewicz, Robert H.
Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain
title Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain
title_full Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain
title_fullStr Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain
title_full_unstemmed Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain
title_short Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain
title_sort genomic and metabolomic insights into the natural product biosynthetic diversity of a feral-hog-associated brevibacillus laterosporus strain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940840/
https://www.ncbi.nlm.nih.gov/pubmed/24595070
http://dx.doi.org/10.1371/journal.pone.0090124
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