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Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients

Oxidative stress involvement has been strongly hypothesized among the possible pathogenic mechanisms of motor neuron degeneration in amyotrophic lateral sclerosis (ALS). The intracellular redox balance is finely modulated by numerous complex mechanisms critical for cellular functions, among which th...

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Autores principales: LoGerfo, Annalisa, Chico, Lucia, Borgia, Loredana, Petrozzi, Lucia, Rocchi, Anna, D'Amelio, Antonia, Carlesi, Cecilia, Caldarazzo Ienco, Elena, Mancuso, Michelangelo, Siciliano, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941162/
https://www.ncbi.nlm.nih.gov/pubmed/24672634
http://dx.doi.org/10.1155/2014/432626
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author LoGerfo, Annalisa
Chico, Lucia
Borgia, Loredana
Petrozzi, Lucia
Rocchi, Anna
D'Amelio, Antonia
Carlesi, Cecilia
Caldarazzo Ienco, Elena
Mancuso, Michelangelo
Siciliano, Gabriele
author_facet LoGerfo, Annalisa
Chico, Lucia
Borgia, Loredana
Petrozzi, Lucia
Rocchi, Anna
D'Amelio, Antonia
Carlesi, Cecilia
Caldarazzo Ienco, Elena
Mancuso, Michelangelo
Siciliano, Gabriele
author_sort LoGerfo, Annalisa
collection PubMed
description Oxidative stress involvement has been strongly hypothesized among the possible pathogenic mechanisms of motor neuron degeneration in amyotrophic lateral sclerosis (ALS). The intracellular redox balance is finely modulated by numerous complex mechanisms critical for cellular functions, among which the nuclear factor erythroid-derived 2-like 2 (NFE2L2/Nrf2) pathways. We genotyped, in a cohort of ALS patients (n = 145) and healthy controls (n = 168), three SNPs in Nrf2 gene promoter: −653 A/G, −651 G/A, and −617 C/A and evaluated, in a subset (n = 73) of patients, advanced oxidation protein products (AOPP), iron-reducing ability of plasma (FRAP), and plasma thiols (-SH) as oxidative damage peripheral biomarkers. Nrf2 polymorphisms were not different among patients and controls. Increased levels of AOPP (P < 0.05) and decreased levels of FRAP (P < 0.001) have been observed in ALS patients compared with controls, but no difference in -SH values was found. Furthermore, no association was found between biochemical markers of redox balance and Nrf2 polymorphisms. These data confirm an altered redox balance in ALS and indicate that, while being abnormally modified compared to controls, the oxidative stress biomarkers assessed in this study are independent from the −653 A/G, −651 G/A, and −617 C/A Nrf2 SNPs in ALS patients.
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spelling pubmed-39411622014-03-26 Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients LoGerfo, Annalisa Chico, Lucia Borgia, Loredana Petrozzi, Lucia Rocchi, Anna D'Amelio, Antonia Carlesi, Cecilia Caldarazzo Ienco, Elena Mancuso, Michelangelo Siciliano, Gabriele Oxid Med Cell Longev Research Article Oxidative stress involvement has been strongly hypothesized among the possible pathogenic mechanisms of motor neuron degeneration in amyotrophic lateral sclerosis (ALS). The intracellular redox balance is finely modulated by numerous complex mechanisms critical for cellular functions, among which the nuclear factor erythroid-derived 2-like 2 (NFE2L2/Nrf2) pathways. We genotyped, in a cohort of ALS patients (n = 145) and healthy controls (n = 168), three SNPs in Nrf2 gene promoter: −653 A/G, −651 G/A, and −617 C/A and evaluated, in a subset (n = 73) of patients, advanced oxidation protein products (AOPP), iron-reducing ability of plasma (FRAP), and plasma thiols (-SH) as oxidative damage peripheral biomarkers. Nrf2 polymorphisms were not different among patients and controls. Increased levels of AOPP (P < 0.05) and decreased levels of FRAP (P < 0.001) have been observed in ALS patients compared with controls, but no difference in -SH values was found. Furthermore, no association was found between biochemical markers of redox balance and Nrf2 polymorphisms. These data confirm an altered redox balance in ALS and indicate that, while being abnormally modified compared to controls, the oxidative stress biomarkers assessed in this study are independent from the −653 A/G, −651 G/A, and −617 C/A Nrf2 SNPs in ALS patients. Hindawi Publishing Corporation 2014 2014-02-09 /pmc/articles/PMC3941162/ /pubmed/24672634 http://dx.doi.org/10.1155/2014/432626 Text en Copyright © 2014 Annalisa LoGerfo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
LoGerfo, Annalisa
Chico, Lucia
Borgia, Loredana
Petrozzi, Lucia
Rocchi, Anna
D'Amelio, Antonia
Carlesi, Cecilia
Caldarazzo Ienco, Elena
Mancuso, Michelangelo
Siciliano, Gabriele
Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients
title Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients
title_full Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients
title_fullStr Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients
title_full_unstemmed Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients
title_short Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients
title_sort lack of association between nuclear factor erythroid-derived 2-like 2 promoter gene polymorphisms and oxidative stress biomarkers in amyotrophic lateral sclerosis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941162/
https://www.ncbi.nlm.nih.gov/pubmed/24672634
http://dx.doi.org/10.1155/2014/432626
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