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Effect of zinc oxide nanoparticles on viability of human spermatozoa
Background: The extensive use of different nanoparticles has raised great concerns about their occupational and biological safety. Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa. Materials and Methods: Semen sam...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research and Clinical Center for Infertility
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941329/ https://www.ncbi.nlm.nih.gov/pubmed/24639818 |
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author | Barkhordari, Abolfazl Hekmatimoghaddam, Seyedhossein Jebali, Ali Khalili, Mohammad Ali Talebi, Alireza Noorani, Marzieh |
author_facet | Barkhordari, Abolfazl Hekmatimoghaddam, Seyedhossein Jebali, Ali Khalili, Mohammad Ali Talebi, Alireza Noorani, Marzieh |
author_sort | Barkhordari, Abolfazl |
collection | PubMed |
description | Background: The extensive use of different nanoparticles has raised great concerns about their occupational and biological safety. Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa. Materials and Methods: Semen samples were obtained from 15 healthy persons, and were analyzed using WHO guidelines. Each semen sample was separately incubated with different concentrations of ZnO NPs (10, 100, 500, and 1000 µg/mL) at 37(o)C for 45, 90, and 180 minutes. Then, the cell death percentage of spermatozoa was measured by MTT assay. Mann-Whitney test was used for comparison of different times and concentrations. Results: The maximum cell death percentage was 20.8%, 21.2%, and 33.2% after 45, 90, and 180 minutes, respectively. In case of concentration, the highest concentration (1000 µg/mL) of ZnO NPs led to the highest toxicity for all incubation times. Statistically, there were significant differences in cell viability after 180 minutes vs. 45 and 90 minutes. Conclusion: This study indicated that cytotoxicity of ZnO NPs is dose and time dependent. This article extracted from M.Sc. thesis. (Marzieh Noorani) |
format | Online Article Text |
id | pubmed-3941329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Research and Clinical Center for Infertility |
record_format | MEDLINE/PubMed |
spelling | pubmed-39413292014-03-17 Effect of zinc oxide nanoparticles on viability of human spermatozoa Barkhordari, Abolfazl Hekmatimoghaddam, Seyedhossein Jebali, Ali Khalili, Mohammad Ali Talebi, Alireza Noorani, Marzieh Iran J Reprod Med Background: The extensive use of different nanoparticles has raised great concerns about their occupational and biological safety. Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa. Materials and Methods: Semen samples were obtained from 15 healthy persons, and were analyzed using WHO guidelines. Each semen sample was separately incubated with different concentrations of ZnO NPs (10, 100, 500, and 1000 µg/mL) at 37(o)C for 45, 90, and 180 minutes. Then, the cell death percentage of spermatozoa was measured by MTT assay. Mann-Whitney test was used for comparison of different times and concentrations. Results: The maximum cell death percentage was 20.8%, 21.2%, and 33.2% after 45, 90, and 180 minutes, respectively. In case of concentration, the highest concentration (1000 µg/mL) of ZnO NPs led to the highest toxicity for all incubation times. Statistically, there were significant differences in cell viability after 180 minutes vs. 45 and 90 minutes. Conclusion: This study indicated that cytotoxicity of ZnO NPs is dose and time dependent. This article extracted from M.Sc. thesis. (Marzieh Noorani) Research and Clinical Center for Infertility 2013-09 /pmc/articles/PMC3941329/ /pubmed/24639818 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Barkhordari, Abolfazl Hekmatimoghaddam, Seyedhossein Jebali, Ali Khalili, Mohammad Ali Talebi, Alireza Noorani, Marzieh Effect of zinc oxide nanoparticles on viability of human spermatozoa |
title | Effect of zinc oxide nanoparticles on viability of human spermatozoa |
title_full | Effect of zinc oxide nanoparticles on viability of human spermatozoa |
title_fullStr | Effect of zinc oxide nanoparticles on viability of human spermatozoa |
title_full_unstemmed | Effect of zinc oxide nanoparticles on viability of human spermatozoa |
title_short | Effect of zinc oxide nanoparticles on viability of human spermatozoa |
title_sort | effect of zinc oxide nanoparticles on viability of human spermatozoa |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941329/ https://www.ncbi.nlm.nih.gov/pubmed/24639818 |
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