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Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos
Background: Embryo cryopreservation is the process that water is removed from the cell by cryoprotectant materials, and embryos are stored at temperature below zero. This process may affect the viability and developmental potential of embryos. Objective: In this study, the effect of the vitrificatio...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research and Clinical Center for Infertility
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941331/ https://www.ncbi.nlm.nih.gov/pubmed/24639813 |
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author | Rajabpour-Niknam, Masoumeh Totonchi, Mehdi Shahhosseini, Maryam Farrokhi, Ali Alipour, Hiva Eftekhari-Yazdi, Poopak |
author_facet | Rajabpour-Niknam, Masoumeh Totonchi, Mehdi Shahhosseini, Maryam Farrokhi, Ali Alipour, Hiva Eftekhari-Yazdi, Poopak |
author_sort | Rajabpour-Niknam, Masoumeh |
collection | PubMed |
description | Background: Embryo cryopreservation is the process that water is removed from the cell by cryoprotectant materials, and embryos are stored at temperature below zero. This process may affect the viability and developmental potential of embryos. Objective: In this study, the effect of the vitrification cryotop method on the expression level of Oct4 and Mest developmental genes in mouse blastocysts was examined. Materials and Methods: The collected 2-cell embryos of superovulated mouse by oviduct flushing were divided into non-vitrified and vitrified groups. These embryos were cultured to the blastocyst stage directly in the non-vitrified group and in the vitrified group, these embryos were cultured to 4-8 cell embryos, vitrified with cryotop in these stages and after 2-6 months, warmed and cultured to blastocyst embryos. Quantitative expression of two developmental genes, namely Oct4 and Mest, were performed in these groups, using RNA purification and Real-time RT-PCR. Results: Quantitative PCR analysis showed that the expression level of both genes, Oct4 and Mest, was reduced significantly in the vitrified-warmed group relative to the control group (p=0.046 and p=0.001). Conclusion: This study revealed that morphologically normal embryos show a reduced amount of Oct4 and Mest transcripts which indicate that the vitrification method negatively effects the expression level of these two developmental genes. |
format | Online Article Text |
id | pubmed-3941331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Research and Clinical Center for Infertility |
record_format | MEDLINE/PubMed |
spelling | pubmed-39413312014-03-17 Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos Rajabpour-Niknam, Masoumeh Totonchi, Mehdi Shahhosseini, Maryam Farrokhi, Ali Alipour, Hiva Eftekhari-Yazdi, Poopak Iran J Reprod Med Background: Embryo cryopreservation is the process that water is removed from the cell by cryoprotectant materials, and embryos are stored at temperature below zero. This process may affect the viability and developmental potential of embryos. Objective: In this study, the effect of the vitrification cryotop method on the expression level of Oct4 and Mest developmental genes in mouse blastocysts was examined. Materials and Methods: The collected 2-cell embryos of superovulated mouse by oviduct flushing were divided into non-vitrified and vitrified groups. These embryos were cultured to the blastocyst stage directly in the non-vitrified group and in the vitrified group, these embryos were cultured to 4-8 cell embryos, vitrified with cryotop in these stages and after 2-6 months, warmed and cultured to blastocyst embryos. Quantitative expression of two developmental genes, namely Oct4 and Mest, were performed in these groups, using RNA purification and Real-time RT-PCR. Results: Quantitative PCR analysis showed that the expression level of both genes, Oct4 and Mest, was reduced significantly in the vitrified-warmed group relative to the control group (p=0.046 and p=0.001). Conclusion: This study revealed that morphologically normal embryos show a reduced amount of Oct4 and Mest transcripts which indicate that the vitrification method negatively effects the expression level of these two developmental genes. Research and Clinical Center for Infertility 2013-09 /pmc/articles/PMC3941331/ /pubmed/24639813 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Rajabpour-Niknam, Masoumeh Totonchi, Mehdi Shahhosseini, Maryam Farrokhi, Ali Alipour, Hiva Eftekhari-Yazdi, Poopak Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos |
title | Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos |
title_full | Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos |
title_fullStr | Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos |
title_full_unstemmed | Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos |
title_short | Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos |
title_sort | quantitative expression of developmental genes, pou5f1 (oct4) and mest (peg1), in vitrified mouse embryos |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941331/ https://www.ncbi.nlm.nih.gov/pubmed/24639813 |
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