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Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination
Significant advances in fluorescence microscopy tend be a balance between two competing qualities wherein improvements in resolution and low light detection are typically accompanied by losses in acquisition rate and signal-to-noise, respectively. These trade-offs are becoming less of a barrier to b...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
YJBM
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941456/ https://www.ncbi.nlm.nih.gov/pubmed/24600334 |
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author | Wolenski, Joseph S. Julich, Doerthe |
author_facet | Wolenski, Joseph S. Julich, Doerthe |
author_sort | Wolenski, Joseph S. |
collection | PubMed |
description | Significant advances in fluorescence microscopy tend be a balance between two competing qualities wherein improvements in resolution and low light detection are typically accompanied by losses in acquisition rate and signal-to-noise, respectively. These trade-offs are becoming less of a barrier to biomedical research as recent advances in optoelectronic microscopy and developments in fluorophore chemistry have enabled scientists to see beyond the diffraction barrier, image deeper into live specimens, and acquire images at unprecedented speed. Selective plane illumination microscopy has provided significant gains in the spatial and temporal acquisition of fluorescence specimens several mm in thickness. With commercial systems now available, this method promises to expand on recent advances in 2-photon deep-tissue imaging with improved speed and reduced photobleaching compared to laser scanning confocal microscopy. Superresolution microscopes are also available in several modalities and can be coupled with selective plane illumination techniques. The combination of methods to increase resolution, acquisition speed, and depth of collection are now being married to common microscope systems, enabling scientists to make significant advances in live cell and in situ imaging in real time. We show that light sheet microscopy provides significant advantages for imaging live zebrafish embryos compared to laser scanning confocal microscopy. |
format | Online Article Text |
id | pubmed-3941456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | YJBM |
record_format | MEDLINE/PubMed |
spelling | pubmed-39414562014-03-05 Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination Wolenski, Joseph S. Julich, Doerthe Yale J Biol Med Focus: Microscopy and Imaging Significant advances in fluorescence microscopy tend be a balance between two competing qualities wherein improvements in resolution and low light detection are typically accompanied by losses in acquisition rate and signal-to-noise, respectively. These trade-offs are becoming less of a barrier to biomedical research as recent advances in optoelectronic microscopy and developments in fluorophore chemistry have enabled scientists to see beyond the diffraction barrier, image deeper into live specimens, and acquire images at unprecedented speed. Selective plane illumination microscopy has provided significant gains in the spatial and temporal acquisition of fluorescence specimens several mm in thickness. With commercial systems now available, this method promises to expand on recent advances in 2-photon deep-tissue imaging with improved speed and reduced photobleaching compared to laser scanning confocal microscopy. Superresolution microscopes are also available in several modalities and can be coupled with selective plane illumination techniques. The combination of methods to increase resolution, acquisition speed, and depth of collection are now being married to common microscope systems, enabling scientists to make significant advances in live cell and in situ imaging in real time. We show that light sheet microscopy provides significant advantages for imaging live zebrafish embryos compared to laser scanning confocal microscopy. YJBM 2014-03-05 /pmc/articles/PMC3941456/ /pubmed/24600334 Text en Copyright ©2014, Yale Journal of Biology and Medicine https://creativecommons.org/licenses/by-nc/3.0/This is an open access article distributed under the terms of the Creative Commons CC BY-NC license, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use the material for commercial purposes. |
spellingShingle | Focus: Microscopy and Imaging Wolenski, Joseph S. Julich, Doerthe Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination |
title | Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination |
title_full | Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination |
title_fullStr | Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination |
title_full_unstemmed | Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination |
title_short | Fluorescence Microscopy Gets Faster and Clearer: Roles of Photochemistry and Selective Illumination |
title_sort | fluorescence microscopy gets faster and clearer: roles of photochemistry and selective illumination |
topic | Focus: Microscopy and Imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941456/ https://www.ncbi.nlm.nih.gov/pubmed/24600334 |
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