What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete

Lyme disease, due to infection with the Ixodes-tick transmitted spirochete Borrelia burgdorferi, is the most common tick-transmitted disease in the northern hemisphere. Our understanding of the tick-pathogen-vertebrate host interactions that sustain an enzootic cycle for B. burgdorferi is incomplete...

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Autores principales: Bockenstedt, Linda K., Gonzalez, David, Mao, Jialing, Li, Ming, Belperron, Alexia A., Haberman, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: YJBM 2014
Materias:
Focus: Microscopy and Imaging
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941458/
https://www.ncbi.nlm.nih.gov/pubmed/24600332
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author Bockenstedt, Linda K.
Gonzalez, David
Mao, Jialing
Li, Ming
Belperron, Alexia A.
Haberman, Ann
author_facet Bockenstedt, Linda K.
Gonzalez, David
Mao, Jialing
Li, Ming
Belperron, Alexia A.
Haberman, Ann
author_sort Bockenstedt, Linda K.
collection PubMed
description Lyme disease, due to infection with the Ixodes-tick transmitted spirochete Borrelia burgdorferi, is the most common tick-transmitted disease in the northern hemisphere. Our understanding of the tick-pathogen-vertebrate host interactions that sustain an enzootic cycle for B. burgdorferi is incomplete. In this article, we describe a method for imaging the feeding of Ixodes scapularis nymphs in real-time using two-photon intravital microscopy and show how this technology can be applied to view the response of Lyme borrelia in the skin of an infected host to tick feeding.
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spelling pubmed-39414582014-03-05 What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete Bockenstedt, Linda K. Gonzalez, David Mao, Jialing Li, Ming Belperron, Alexia A. Haberman, Ann Yale J Biol Med Focus: Microscopy and Imaging Lyme disease, due to infection with the Ixodes-tick transmitted spirochete Borrelia burgdorferi, is the most common tick-transmitted disease in the northern hemisphere. Our understanding of the tick-pathogen-vertebrate host interactions that sustain an enzootic cycle for B. burgdorferi is incomplete. In this article, we describe a method for imaging the feeding of Ixodes scapularis nymphs in real-time using two-photon intravital microscopy and show how this technology can be applied to view the response of Lyme borrelia in the skin of an infected host to tick feeding. YJBM 2014-03-05 /pmc/articles/PMC3941458/ /pubmed/24600332 Text en Copyright ©2014, Yale Journal of Biology and Medicine https://creativecommons.org/licenses/by-nc/3.0/This is an open access article distributed under the terms of the Creative Commons CC BY-NC license, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use the material for commercial purposes.
spellingShingle Focus: Microscopy and Imaging
Bockenstedt, Linda K.
Gonzalez, David
Mao, Jialing
Li, Ming
Belperron, Alexia A.
Haberman, Ann
What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete
title What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete
title_full What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete
title_fullStr What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete
title_full_unstemmed What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete
title_short What Ticks Do Under Your Skin: Two-Photon Intravital Imaging of Ixodes Scapularis Feeding in the Presence of the Lyme Disease Spirochete
title_sort what ticks do under your skin: two-photon intravital imaging of ixodes scapularis feeding in the presence of the lyme disease spirochete
topic Focus: Microscopy and Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941458/
https://www.ncbi.nlm.nih.gov/pubmed/24600332
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