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Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse

Background. To evaluate the correlation between ERG, OCT, and microscopic findings in the rd10 mouse. Methods. C57BL/6J wild type mice and rd10 mice were compared at the age of 2, 3, 5, 7, 9, 12, 24, and 48 weeks (each age group n = 3) using full-field electroretinography (ERG), spectral domain Opti...

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Autores principales: Rösch, Sarah, Johnen, Sandra, Müller, Frank, Pfarrer, Christiane, Walter, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941775/
https://www.ncbi.nlm.nih.gov/pubmed/24683495
http://dx.doi.org/10.1155/2014/874751
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author Rösch, Sarah
Johnen, Sandra
Müller, Frank
Pfarrer, Christiane
Walter, Peter
author_facet Rösch, Sarah
Johnen, Sandra
Müller, Frank
Pfarrer, Christiane
Walter, Peter
author_sort Rösch, Sarah
collection PubMed
description Background. To evaluate the correlation between ERG, OCT, and microscopic findings in the rd10 mouse. Methods. C57BL/6J wild type mice and rd10 mice were compared at the age of 2, 3, 5, 7, 9, 12, 24, and 48 weeks (each age group n = 3) using full-field electroretinography (ERG), spectral domain Optical Coherence Tomography (sd-OCT), fluorescein angiography (FA), Hematoxylin & Eosin histology (HE), and immunohistology (IH). Results. While in wild type mice, the amplitude of a- and b-wave increased with light intensity and with the age of the animals, the rd10 mice showed extinction of the ERG beginning with the age of 5 weeks. In OCT recordings, the thickness of the retina decreased up to 9 weeks of age, mainly based on the degradation of the outer nuclear layer (ONL). Afterwards, the ONL was no longer visible in the OCT. HE staining and immunohistological findings confirmed the in vivo data. Conclusion. ERG and OCT are useful methods to evaluate the retinal function and structure in vivo. The retinal changes seen in the OCT closely match those observed in histological staining.
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spelling pubmed-39417752014-03-30 Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse Rösch, Sarah Johnen, Sandra Müller, Frank Pfarrer, Christiane Walter, Peter J Ophthalmol Research Article Background. To evaluate the correlation between ERG, OCT, and microscopic findings in the rd10 mouse. Methods. C57BL/6J wild type mice and rd10 mice were compared at the age of 2, 3, 5, 7, 9, 12, 24, and 48 weeks (each age group n = 3) using full-field electroretinography (ERG), spectral domain Optical Coherence Tomography (sd-OCT), fluorescein angiography (FA), Hematoxylin & Eosin histology (HE), and immunohistology (IH). Results. While in wild type mice, the amplitude of a- and b-wave increased with light intensity and with the age of the animals, the rd10 mice showed extinction of the ERG beginning with the age of 5 weeks. In OCT recordings, the thickness of the retina decreased up to 9 weeks of age, mainly based on the degradation of the outer nuclear layer (ONL). Afterwards, the ONL was no longer visible in the OCT. HE staining and immunohistological findings confirmed the in vivo data. Conclusion. ERG and OCT are useful methods to evaluate the retinal function and structure in vivo. The retinal changes seen in the OCT closely match those observed in histological staining. Hindawi Publishing Corporation 2014 2014-01-19 /pmc/articles/PMC3941775/ /pubmed/24683495 http://dx.doi.org/10.1155/2014/874751 Text en Copyright © 2014 Sarah Rösch et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rösch, Sarah
Johnen, Sandra
Müller, Frank
Pfarrer, Christiane
Walter, Peter
Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
title Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
title_full Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
title_fullStr Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
title_full_unstemmed Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
title_short Correlations between ERG, OCT, and Anatomical Findings in the rd10 Mouse
title_sort correlations between erg, oct, and anatomical findings in the rd10 mouse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941775/
https://www.ncbi.nlm.nih.gov/pubmed/24683495
http://dx.doi.org/10.1155/2014/874751
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