The Evaluation of Antibacterial, Antifungal and Antioxidant Activity of Methanolic Extract of Mindium Laevigatum (Vent.) Rech. F., From Central Part of Iran

BACKGROUND: Mindium laevigatum (Vent.) Rech. F. plant grows in central part of Iran. And used by local people as medical plant. OBJECTIVES: The purpose of this study was to investigate the in vitro antibacterial, antifungal and antioxidant activities of the methanolic extracts of aerial and flower parts of plant. MATERIALS AND METHODS: The leaves and stem and flower of bark from M. laevigatum were separately collected, air-dried and powdered. Then the plant species extracts were prepared with methanol, water 80:20 and two polar and non-polar subfractions were realized. The antioxidant activity was evaluated by scavenging the radicals 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), β-Carotene linoleic acid assay and reducing power methods. The antifungal and antibacterial evaluation was performed by disc diffusion and minimum inhibitory concentration methods. RESULTS: The total phenolic analysis of subfractions found 182 ± 4.2 µg.gr(-1) for polar and 158 ± 3.9 µg.gr(-1) for non-polar extracts. The antifungal activity of the extracts against the various fungal varied from 14.0 to 34 mm. MIC values from 50 to 400 µg.mL(-1) were satisfactory when compared with other plant products. The antibacterial results revealed that the subfraction extracts are mostly effective against Staphylococcus aureus. The antioxidant results showed polar subfraction has more activity against non-polar subfraction. CONCLUSION: These findings demonstrated that the extract of Mindium laevigatum has remarkable in vitro antifungal and antioxidant activity.