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Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation

Amylase is an enzyme that catalyzes the breakdown of starch into sugars and plays a pivotal role in a variety of areas like use as digestives, for the production of ethanol and high fructose corn syrup, detergents, desiring of textiles, modified starches, hydrolysis of oil-field drilling fluids, and...

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Autores principales: Raul, Dibyangana, Biswas, Tania, Mukhopadhyay, Suchita, Kumar Das, Shrayan, Gupta, Suvroma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942096/
https://www.ncbi.nlm.nih.gov/pubmed/24672727
http://dx.doi.org/10.1155/2014/568141
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author Raul, Dibyangana
Biswas, Tania
Mukhopadhyay, Suchita
Kumar Das, Shrayan
Gupta, Suvroma
author_facet Raul, Dibyangana
Biswas, Tania
Mukhopadhyay, Suchita
Kumar Das, Shrayan
Gupta, Suvroma
author_sort Raul, Dibyangana
collection PubMed
description Amylase is an enzyme that catalyzes the breakdown of starch into sugars and plays a pivotal role in a variety of areas like use as digestives, for the production of ethanol and high fructose corn syrup, detergents, desiring of textiles, modified starches, hydrolysis of oil-field drilling fluids, and paper recycling. In the present work, solid state fermentation (SSF) for α-amylase production has been used in lieu of submerged fermentation (SmF) due to its simple technique, low capital investment, lower levels of catabolite repression, and better product recovery. Bacillus subtilis has been well known as producer of alpha amylase and was tested using solid state fermentation for 48 hours at 37°C with wheat bran as substrate. Comparison between different fermentation hours demonstrated high yield of alpha amylase after 48 hours. This alpha amylase has optimum pH and temperature at 7.1 and 40°C, respectively. With the goal to purify alpha amylase, 30–70% (NH(4))(2)SO(4) cut concentrated the amylase activity threefold with respect to crude fermented extract. This was verified in quantitative DNS assay method as well as in zymogram gel profile. The exact molecular weight of the amylase is yet to be determined with the aid of other protein purification techniques.
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spelling pubmed-39420962014-03-26 Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation Raul, Dibyangana Biswas, Tania Mukhopadhyay, Suchita Kumar Das, Shrayan Gupta, Suvroma Biochem Res Int Research Article Amylase is an enzyme that catalyzes the breakdown of starch into sugars and plays a pivotal role in a variety of areas like use as digestives, for the production of ethanol and high fructose corn syrup, detergents, desiring of textiles, modified starches, hydrolysis of oil-field drilling fluids, and paper recycling. In the present work, solid state fermentation (SSF) for α-amylase production has been used in lieu of submerged fermentation (SmF) due to its simple technique, low capital investment, lower levels of catabolite repression, and better product recovery. Bacillus subtilis has been well known as producer of alpha amylase and was tested using solid state fermentation for 48 hours at 37°C with wheat bran as substrate. Comparison between different fermentation hours demonstrated high yield of alpha amylase after 48 hours. This alpha amylase has optimum pH and temperature at 7.1 and 40°C, respectively. With the goal to purify alpha amylase, 30–70% (NH(4))(2)SO(4) cut concentrated the amylase activity threefold with respect to crude fermented extract. This was verified in quantitative DNS assay method as well as in zymogram gel profile. The exact molecular weight of the amylase is yet to be determined with the aid of other protein purification techniques. Hindawi Publishing Corporation 2014 2014-01-12 /pmc/articles/PMC3942096/ /pubmed/24672727 http://dx.doi.org/10.1155/2014/568141 Text en Copyright © 2014 Dibyangana Raul et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Raul, Dibyangana
Biswas, Tania
Mukhopadhyay, Suchita
Kumar Das, Shrayan
Gupta, Suvroma
Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
title Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
title_full Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
title_fullStr Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
title_full_unstemmed Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
title_short Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation
title_sort production and partial purification of alpha amylase from bacillus subtilis (mtcc 121) using solid state fermentation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942096/
https://www.ncbi.nlm.nih.gov/pubmed/24672727
http://dx.doi.org/10.1155/2014/568141
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