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Quality assessment and control of tissue specific RNA-seq libraries of Drosophila transgenic RNAi models

RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq is not a bias free technique. It requires large amounts of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages in the process....

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Detalles Bibliográficos
Autores principales: Amaral, Andreia J., Brito, Francisco F., Chobanyan, Tamar, Yoshikawa, Seiko, Yokokura, Takakazu, Van Vactor, David, Gama-Carvalho, Margarida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942661/
https://www.ncbi.nlm.nih.gov/pubmed/24634674
http://dx.doi.org/10.3389/fgene.2014.00043

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