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Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease
BACKGROUND: Inflammatory bowel disease constitutes a heterogeneous group of conditions, whose aetiology is only partly understood. The prevailing hypothesis on its pathogenesis is that IBD is the result of an inadequate immune response to the resident bacterial flora of the intestine. An autoimmune...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942769/ https://www.ncbi.nlm.nih.gov/pubmed/24581234 http://dx.doi.org/10.1186/1471-2172-15-10 |
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author | Frede, Natalie Glocker, Erik-Oliver Wanders, Jennifer Engelhardt, Karin R Kreisel, Wolfgang Ruemmele, Frank M Grimbacher, Bodo |
author_facet | Frede, Natalie Glocker, Erik-Oliver Wanders, Jennifer Engelhardt, Karin R Kreisel, Wolfgang Ruemmele, Frank M Grimbacher, Bodo |
author_sort | Frede, Natalie |
collection | PubMed |
description | BACKGROUND: Inflammatory bowel disease constitutes a heterogeneous group of conditions, whose aetiology is only partly understood. The prevailing hypothesis on its pathogenesis is that IBD is the result of an inadequate immune response to the resident bacterial flora of the intestine. An autoimmune background, however, has been discussed since the 1950s. Lately, it has been shown that failures in interleukin-10 (IL-10) signalling due to IL-10- and IL-10 receptor (IL-10R) mutations result in IBD. Our study aimed at investigating the existence of inhibitory autoantibodies against IL-10 and IL-10R in IBD patients capable of down-modulating IL-10 signalling thereby mimicking IL-10 or IL-10R deficiency. RESULTS: Thirteen IBD patients had IgG autoantibodies against IL-10, IL-10RA and/or IL-10RB, and three patients had IgA autoantibodies against IL-10. However, the absolute OD values of the serum antibodies measured by ELISA were low, there was overall no significant difference between patients and controls, and positive sera had no neutralizing activity. CONCLUSION: No evidence for an involvement of autoantibodies against IL-10 or IL-10R in the pathogenesis of inflammatory bowel disease could be established. |
format | Online Article Text |
id | pubmed-3942769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39427692014-03-06 Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease Frede, Natalie Glocker, Erik-Oliver Wanders, Jennifer Engelhardt, Karin R Kreisel, Wolfgang Ruemmele, Frank M Grimbacher, Bodo BMC Immunol Research Article BACKGROUND: Inflammatory bowel disease constitutes a heterogeneous group of conditions, whose aetiology is only partly understood. The prevailing hypothesis on its pathogenesis is that IBD is the result of an inadequate immune response to the resident bacterial flora of the intestine. An autoimmune background, however, has been discussed since the 1950s. Lately, it has been shown that failures in interleukin-10 (IL-10) signalling due to IL-10- and IL-10 receptor (IL-10R) mutations result in IBD. Our study aimed at investigating the existence of inhibitory autoantibodies against IL-10 and IL-10R in IBD patients capable of down-modulating IL-10 signalling thereby mimicking IL-10 or IL-10R deficiency. RESULTS: Thirteen IBD patients had IgG autoantibodies against IL-10, IL-10RA and/or IL-10RB, and three patients had IgA autoantibodies against IL-10. However, the absolute OD values of the serum antibodies measured by ELISA were low, there was overall no significant difference between patients and controls, and positive sera had no neutralizing activity. CONCLUSION: No evidence for an involvement of autoantibodies against IL-10 or IL-10R in the pathogenesis of inflammatory bowel disease could be established. BioMed Central 2014-02-28 /pmc/articles/PMC3942769/ /pubmed/24581234 http://dx.doi.org/10.1186/1471-2172-15-10 Text en Copyright © 2014 Frede et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Article Frede, Natalie Glocker, Erik-Oliver Wanders, Jennifer Engelhardt, Karin R Kreisel, Wolfgang Ruemmele, Frank M Grimbacher, Bodo Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease |
title | Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease |
title_full | Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease |
title_fullStr | Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease |
title_full_unstemmed | Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease |
title_short | Evidence for non-neutralizing autoantibodies against IL-10 signalling components in patients with inflammatory bowel disease |
title_sort | evidence for non-neutralizing autoantibodies against il-10 signalling components in patients with inflammatory bowel disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942769/ https://www.ncbi.nlm.nih.gov/pubmed/24581234 http://dx.doi.org/10.1186/1471-2172-15-10 |
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