Correlative super-resolution fluorescence and metal replica transmission electron microscopy

Super-resolution localization microscopy is combined with a complementary imaging technique, transmission electron microscopy of metal replicas, to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. Robust correlation on the scale of 20 nm is validated by imaging endo...

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Detalles Bibliográficos
Autores principales: Sochacki, Kem A., Shtengel, Gleb, van Engelenburg, Schuyler B., Hess, Harald F., Taraska, Justin W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943662/
https://www.ncbi.nlm.nih.gov/pubmed/24464288
http://dx.doi.org/10.1038/nmeth.2816
Descripción
Sumario:Super-resolution localization microscopy is combined with a complementary imaging technique, transmission electron microscopy of metal replicas, to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. Robust correlation on the scale of 20 nm is validated by imaging endogenous clathrin (with 2D and 3D PALM/TEM) and the method is further used to find the previously unknown 3D position of epsin on clathrin coated structures.

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