Correlative super-resolution fluorescence and metal replica transmission electron microscopy

Super-resolution localization microscopy is combined with a complementary imaging technique, transmission electron microscopy of metal replicas, to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. Robust correlation on the scale of 20 nm is validated by imaging endo...

Descripción completa

Detalles Bibliográficos
Autores principales: Sochacki, Kem A., Shtengel, Gleb, van Engelenburg, Schuyler B., Hess, Harald F., Taraska, Justin W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943662/
https://www.ncbi.nlm.nih.gov/pubmed/24464288
http://dx.doi.org/10.1038/nmeth.2816
_version_ 1782306291213926400
author Sochacki, Kem A.
Shtengel, Gleb
van Engelenburg, Schuyler B.
Hess, Harald F.
Taraska, Justin W.
author_facet Sochacki, Kem A.
Shtengel, Gleb
van Engelenburg, Schuyler B.
Hess, Harald F.
Taraska, Justin W.
author_sort Sochacki, Kem A.
collection PubMed
description Super-resolution localization microscopy is combined with a complementary imaging technique, transmission electron microscopy of metal replicas, to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. Robust correlation on the scale of 20 nm is validated by imaging endogenous clathrin (with 2D and 3D PALM/TEM) and the method is further used to find the previously unknown 3D position of epsin on clathrin coated structures.
format Online
Article
Text
id pubmed-3943662
institution National Center for Biotechnology Information
language English
publishDate 2014
record_format MEDLINE/PubMed
spelling pubmed-39436622014-09-01 Correlative super-resolution fluorescence and metal replica transmission electron microscopy Sochacki, Kem A. Shtengel, Gleb van Engelenburg, Schuyler B. Hess, Harald F. Taraska, Justin W. Nat Methods Article Super-resolution localization microscopy is combined with a complementary imaging technique, transmission electron microscopy of metal replicas, to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. Robust correlation on the scale of 20 nm is validated by imaging endogenous clathrin (with 2D and 3D PALM/TEM) and the method is further used to find the previously unknown 3D position of epsin on clathrin coated structures. 2014-01-26 2014-03 /pmc/articles/PMC3943662/ /pubmed/24464288 http://dx.doi.org/10.1038/nmeth.2816 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sochacki, Kem A.
Shtengel, Gleb
van Engelenburg, Schuyler B.
Hess, Harald F.
Taraska, Justin W.
Correlative super-resolution fluorescence and metal replica transmission electron microscopy
title Correlative super-resolution fluorescence and metal replica transmission electron microscopy
title_full Correlative super-resolution fluorescence and metal replica transmission electron microscopy
title_fullStr Correlative super-resolution fluorescence and metal replica transmission electron microscopy
title_full_unstemmed Correlative super-resolution fluorescence and metal replica transmission electron microscopy
title_short Correlative super-resolution fluorescence and metal replica transmission electron microscopy
title_sort correlative super-resolution fluorescence and metal replica transmission electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943662/
https://www.ncbi.nlm.nih.gov/pubmed/24464288
http://dx.doi.org/10.1038/nmeth.2816
work_keys_str_mv AT sochackikema correlativesuperresolutionfluorescenceandmetalreplicatransmissionelectronmicroscopy
AT shtengelgleb correlativesuperresolutionfluorescenceandmetalreplicatransmissionelectronmicroscopy
AT vanengelenburgschuylerb correlativesuperresolutionfluorescenceandmetalreplicatransmissionelectronmicroscopy
AT hessharaldf correlativesuperresolutionfluorescenceandmetalreplicatransmissionelectronmicroscopy
AT taraskajustinw correlativesuperresolutionfluorescenceandmetalreplicatransmissionelectronmicroscopy

Ejemplares similares