Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9

The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (A...

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Detalles Bibliográficos
Autores principales: Ma, Yuanwu, Shen, Bin, Zhang, Xu, Lu, Yingdong, Chen, Wei, Ma, Jing, Huang, Xingxu, Zhang, Lianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943732/
https://www.ncbi.nlm.nih.gov/pubmed/24598943
http://dx.doi.org/10.1371/journal.pone.0089413
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author Ma, Yuanwu
Shen, Bin
Zhang, Xu
Lu, Yingdong
Chen, Wei
Ma, Jing
Huang, Xingxu
Zhang, Lianfeng
author_facet Ma, Yuanwu
Shen, Bin
Zhang, Xu
Lu, Yingdong
Chen, Wei
Ma, Jing
Huang, Xingxu
Zhang, Lianfeng
author_sort Ma, Yuanwu
collection PubMed
description The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (ApoE, B2m, Prf1, and Prkdc) in rats in one-step, by co-injection of Cas9 mRNA and sgRNAs into fertilized eggs. We further observed the gene modifications are germline transmittable, and confirmed the off-target mutagenesis and mosaicism are rarely detected by comprehensive analysis. Thus, the CRISPR/Cas9 system makes it possible to efficiently and reliably generate gene knock-out rats.
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spelling pubmed-39437322014-03-10 Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9 Ma, Yuanwu Shen, Bin Zhang, Xu Lu, Yingdong Chen, Wei Ma, Jing Huang, Xingxu Zhang, Lianfeng PLoS One Research Article The CRISPR/Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (ApoE, B2m, Prf1, and Prkdc) in rats in one-step, by co-injection of Cas9 mRNA and sgRNAs into fertilized eggs. We further observed the gene modifications are germline transmittable, and confirmed the off-target mutagenesis and mosaicism are rarely detected by comprehensive analysis. Thus, the CRISPR/Cas9 system makes it possible to efficiently and reliably generate gene knock-out rats. Public Library of Science 2014-03-05 /pmc/articles/PMC3943732/ /pubmed/24598943 http://dx.doi.org/10.1371/journal.pone.0089413 Text en © 2014 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ma, Yuanwu
Shen, Bin
Zhang, Xu
Lu, Yingdong
Chen, Wei
Ma, Jing
Huang, Xingxu
Zhang, Lianfeng
Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
title Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
title_full Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
title_fullStr Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
title_full_unstemmed Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
title_short Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
title_sort heritable multiplex genetic engineering in rats using crispr/cas9
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943732/
https://www.ncbi.nlm.nih.gov/pubmed/24598943
http://dx.doi.org/10.1371/journal.pone.0089413
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