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Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence

Localization patterns of lipid droplets in the cytoplasm of porcine oocytes were evaluated as a novel marker for in vitro maturation (IVM) of oocytes with high developmental competence. Porcine oocytes were cultured in TCM-199, which is a complete synthetic medium, for 44 h at 38.5 C. Localization p...

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Autores principales: HIRAGA, Kou, HOSHINO, Yumi, TANEMURA, Kentaro, SATO, Eimei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944360/
https://www.ncbi.nlm.nih.gov/pubmed/23594924
http://dx.doi.org/10.1262/jrd.2012-126
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author HIRAGA, Kou
HOSHINO, Yumi
TANEMURA, Kentaro
SATO, Eimei
author_facet HIRAGA, Kou
HOSHINO, Yumi
TANEMURA, Kentaro
SATO, Eimei
author_sort HIRAGA, Kou
collection PubMed
description Localization patterns of lipid droplets in the cytoplasm of porcine oocytes were evaluated as a novel marker for in vitro maturation (IVM) of oocytes with high developmental competence. Porcine oocytes were cultured in TCM-199, which is a complete synthetic medium, for 44 h at 38.5 C. Localization patterns were divided into 2 classes: lipid droplets localized uniformly in the whole cytoplasm (class I) and those that were centrally located (class II). After IVM in TCM-199, 60% of matured oocytes exhibited the class II pattern. To investigate the relation between the distribution of lipid droplets and the developmental rate of the oocyte, the developmental rates of class I and class II oocytes were compared after in vitro fertilization (IVF). Class II oocytes showed a significantly higher rate of blastocyst development than class I oocytes. These results suggest that porcine oocytes with high developmental competence can be selected based on the localization patterns of lipid droplets.
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spelling pubmed-39443602014-03-06 Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence HIRAGA, Kou HOSHINO, Yumi TANEMURA, Kentaro SATO, Eimei J Reprod Dev Technology Report Localization patterns of lipid droplets in the cytoplasm of porcine oocytes were evaluated as a novel marker for in vitro maturation (IVM) of oocytes with high developmental competence. Porcine oocytes were cultured in TCM-199, which is a complete synthetic medium, for 44 h at 38.5 C. Localization patterns were divided into 2 classes: lipid droplets localized uniformly in the whole cytoplasm (class I) and those that were centrally located (class II). After IVM in TCM-199, 60% of matured oocytes exhibited the class II pattern. To investigate the relation between the distribution of lipid droplets and the developmental rate of the oocyte, the developmental rates of class I and class II oocytes were compared after in vitro fertilization (IVF). Class II oocytes showed a significantly higher rate of blastocyst development than class I oocytes. These results suggest that porcine oocytes with high developmental competence can be selected based on the localization patterns of lipid droplets. The Society for Reproduction and Development 2013-04-18 2013-08 /pmc/articles/PMC3944360/ /pubmed/23594924 http://dx.doi.org/10.1262/jrd.2012-126 Text en ©2013 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Technology Report
HIRAGA, Kou
HOSHINO, Yumi
TANEMURA, Kentaro
SATO, Eimei
Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence
title Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence
title_full Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence
title_fullStr Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence
title_full_unstemmed Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence
title_short Selection of In Vitro-Matured Porcine Oocytes Based on Localization Patterns of Lipid Droplets to Evaluate Developmental Competence
title_sort selection of in vitro-matured porcine oocytes based on localization patterns of lipid droplets to evaluate developmental competence
topic Technology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944360/
https://www.ncbi.nlm.nih.gov/pubmed/23594924
http://dx.doi.org/10.1262/jrd.2012-126
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