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Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada

Rectal swabs from 155 sheep and 252 goats from Grenada were evaluated to determine the prevalence of Campylobacter spp., antibiotic resistance, and multilocus sequence types. Fifteen Campylobacter isolates were obtained (14 C. jejuni and 1 C. coli). The prevalence (3.7%) did not differ significantly...

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Autores principales: Stone, Diana M., Chander, Yogesh, Bekele, Aschalew Z., Goyal, Sagar M., Hariharan, Harry, Tiwari, Keshaw, Chikweto, Alfred, Sharma, Ravindra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944391/
https://www.ncbi.nlm.nih.gov/pubmed/24693459
http://dx.doi.org/10.1155/2014/212864
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author Stone, Diana M.
Chander, Yogesh
Bekele, Aschalew Z.
Goyal, Sagar M.
Hariharan, Harry
Tiwari, Keshaw
Chikweto, Alfred
Sharma, Ravindra
author_facet Stone, Diana M.
Chander, Yogesh
Bekele, Aschalew Z.
Goyal, Sagar M.
Hariharan, Harry
Tiwari, Keshaw
Chikweto, Alfred
Sharma, Ravindra
author_sort Stone, Diana M.
collection PubMed
description Rectal swabs from 155 sheep and 252 goats from Grenada were evaluated to determine the prevalence of Campylobacter spp., antibiotic resistance, and multilocus sequence types. Fifteen Campylobacter isolates were obtained (14 C. jejuni and 1 C. coli). The prevalence (3.7%) did not differ significantly between sheep (4.5%) and goats (3.2%). Among the seven antimicrobials tested, resistance was only detected for tetracycline (30.8%) and metronidazole (38.5%). Campylobacter isolates showed no significant difference between sheep and goats for type of antimicrobial resistance or percent of resistant isolates. Twelve of the isolates were successfully genotyped consisting of four recognized clonal complexes and three novel sequence types. Importantly, one isolate from one goat was identified as the C. jejuni sequence type-8, a zoonotic and tetracycline-resistant clone reported to be a highly virulent clone associated with ovine abortion in the USA. Although most samples were from comingled sheep and goat production units, there were no shared sequence types between these two host species. None of the sequence types identified in this study have previously been reported in poultry in Grenada, suggesting sheep- and goat-specific Campylobacter clones in Grenada. This is the first report of genotyping of Campylobacter isolates from sheep and goats in the Eastern Caribbean.
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spelling pubmed-39443912014-04-01 Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada Stone, Diana M. Chander, Yogesh Bekele, Aschalew Z. Goyal, Sagar M. Hariharan, Harry Tiwari, Keshaw Chikweto, Alfred Sharma, Ravindra Vet Med Int Research Article Rectal swabs from 155 sheep and 252 goats from Grenada were evaluated to determine the prevalence of Campylobacter spp., antibiotic resistance, and multilocus sequence types. Fifteen Campylobacter isolates were obtained (14 C. jejuni and 1 C. coli). The prevalence (3.7%) did not differ significantly between sheep (4.5%) and goats (3.2%). Among the seven antimicrobials tested, resistance was only detected for tetracycline (30.8%) and metronidazole (38.5%). Campylobacter isolates showed no significant difference between sheep and goats for type of antimicrobial resistance or percent of resistant isolates. Twelve of the isolates were successfully genotyped consisting of four recognized clonal complexes and three novel sequence types. Importantly, one isolate from one goat was identified as the C. jejuni sequence type-8, a zoonotic and tetracycline-resistant clone reported to be a highly virulent clone associated with ovine abortion in the USA. Although most samples were from comingled sheep and goat production units, there were no shared sequence types between these two host species. None of the sequence types identified in this study have previously been reported in poultry in Grenada, suggesting sheep- and goat-specific Campylobacter clones in Grenada. This is the first report of genotyping of Campylobacter isolates from sheep and goats in the Eastern Caribbean. Hindawi Publishing Corporation 2014 2014-02-11 /pmc/articles/PMC3944391/ /pubmed/24693459 http://dx.doi.org/10.1155/2014/212864 Text en Copyright © 2014 Diana M. Stone et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Stone, Diana M.
Chander, Yogesh
Bekele, Aschalew Z.
Goyal, Sagar M.
Hariharan, Harry
Tiwari, Keshaw
Chikweto, Alfred
Sharma, Ravindra
Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
title Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
title_full Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
title_fullStr Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
title_full_unstemmed Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
title_short Genotypes, Antibiotic Resistance, and ST-8 Genetic Clone in Campylobacter Isolates from Sheep and Goats in Grenada
title_sort genotypes, antibiotic resistance, and st-8 genetic clone in campylobacter isolates from sheep and goats in grenada
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944391/
https://www.ncbi.nlm.nih.gov/pubmed/24693459
http://dx.doi.org/10.1155/2014/212864
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