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The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages
The control of self-renewal and differentiation of neural stem and progenitor cells is a crucial issue in stem cell and cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired neuroblast homeostasis if the limited self-renewing potential of intermediate neural progeni...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944433/ https://www.ncbi.nlm.nih.gov/pubmed/24618901 http://dx.doi.org/10.7554/eLife.01906 |
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author | Koe, Chwee Tat Li, Song Rossi, Fabrizio Wong, Jack Jing Lin Wang, Yan Zhang, Zhizhuo Chen, Keng Aw, Sherry Shiying Richardson, Helena E Robson, Paul Sung, Wing-Kin Yu, Fengwei Gonzalez, Cayetano Wang, Hongyan |
author_facet | Koe, Chwee Tat Li, Song Rossi, Fabrizio Wong, Jack Jing Lin Wang, Yan Zhang, Zhizhuo Chen, Keng Aw, Sherry Shiying Richardson, Helena E Robson, Paul Sung, Wing-Kin Yu, Fengwei Gonzalez, Cayetano Wang, Hongyan |
author_sort | Koe, Chwee Tat |
collection | PubMed |
description | The control of self-renewal and differentiation of neural stem and progenitor cells is a crucial issue in stem cell and cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired neuroblast homeostasis if the limited self-renewing potential of intermediate neural progenitors (INPs) is unrestrained. Here, we demonstrate that Drosophila SWI/SNF chromatin remodeling Brahma (Brm) complex functions cooperatively with another chromatin remodeling factor, Histone deacetylase 3 (HDAC3) to suppress the formation of ectopic type II neuroblasts. We show that multiple components of the Brm complex and HDAC3 physically associate with Earmuff (Erm), a type II-specific transcription factor that prevents dedifferentiation of INPs into neuroblasts. Consistently, the predicted Erm-binding motif is present in most of known binding loci of Brm. Furthermore, brm and hdac3 genetically interact with erm to prevent type II neuroblast overgrowth. Thus, the Brm-HDAC3-Erm repressor complex suppresses dedifferentiation of INPs back into type II neuroblasts. DOI: http://dx.doi.org/10.7554/eLife.01906.001 |
format | Online Article Text |
id | pubmed-3944433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-39444332014-03-13 The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages Koe, Chwee Tat Li, Song Rossi, Fabrizio Wong, Jack Jing Lin Wang, Yan Zhang, Zhizhuo Chen, Keng Aw, Sherry Shiying Richardson, Helena E Robson, Paul Sung, Wing-Kin Yu, Fengwei Gonzalez, Cayetano Wang, Hongyan eLife Developmental Biology and Stem Cells The control of self-renewal and differentiation of neural stem and progenitor cells is a crucial issue in stem cell and cancer biology. Drosophila type II neuroblast lineages are prone to developing impaired neuroblast homeostasis if the limited self-renewing potential of intermediate neural progenitors (INPs) is unrestrained. Here, we demonstrate that Drosophila SWI/SNF chromatin remodeling Brahma (Brm) complex functions cooperatively with another chromatin remodeling factor, Histone deacetylase 3 (HDAC3) to suppress the formation of ectopic type II neuroblasts. We show that multiple components of the Brm complex and HDAC3 physically associate with Earmuff (Erm), a type II-specific transcription factor that prevents dedifferentiation of INPs into neuroblasts. Consistently, the predicted Erm-binding motif is present in most of known binding loci of Brm. Furthermore, brm and hdac3 genetically interact with erm to prevent type II neuroblast overgrowth. Thus, the Brm-HDAC3-Erm repressor complex suppresses dedifferentiation of INPs back into type II neuroblasts. DOI: http://dx.doi.org/10.7554/eLife.01906.001 eLife Sciences Publications, Ltd 2014-03-11 /pmc/articles/PMC3944433/ /pubmed/24618901 http://dx.doi.org/10.7554/eLife.01906 Text en Copyright © 2014, Koe et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Koe, Chwee Tat Li, Song Rossi, Fabrizio Wong, Jack Jing Lin Wang, Yan Zhang, Zhizhuo Chen, Keng Aw, Sherry Shiying Richardson, Helena E Robson, Paul Sung, Wing-Kin Yu, Fengwei Gonzalez, Cayetano Wang, Hongyan The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
title | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
title_full | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
title_fullStr | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
title_full_unstemmed | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
title_short | The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in Drosophila type II neuroblast lineages |
title_sort | brm-hdac3-erm repressor complex suppresses dedifferentiation in drosophila type ii neuroblast lineages |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944433/ https://www.ncbi.nlm.nih.gov/pubmed/24618901 http://dx.doi.org/10.7554/eLife.01906 |
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