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Chlorogenic Acid Inhibits Human Platelet Activation and Thrombus Formation

BACKGROUND: Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were examined. METHODS AND...

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Detalles Bibliográficos
Autores principales: Fuentes, Eduardo, Caballero, Julio, Alarcón, Marcelo, Rojas, Armando, Palomo, Iván
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944540/
https://www.ncbi.nlm.nih.gov/pubmed/24598787
http://dx.doi.org/10.1371/journal.pone.0090699
Descripción
Sumario:BACKGROUND: Chlorogenic acid is a potent phenolic antioxidant. However, its effect on platelet aggregation, a critical factor in arterial thrombosis, remains unclear. Consequently, chlorogenic acid-action mechanisms in preventing platelet activation and thrombus formation were examined. METHODS AND RESULTS: Chlorogenic acid in a dose-dependent manner (0.1 to 1 mmol/L) inhibited platelet secretion and aggregation induced by ADP, collagen, arachidonic acid and TRAP-6, and diminished platelet firm adhesion/aggregation and platelet-leukocyte interactions under flow conditions. At these concentrations chlorogenic acid significantly decreased platelet inflammatory mediators (sP-selectin, sCD40L, CCL5 and IL-1β) and increased intraplatelet cAMP levels/PKA activation. Interestingly, SQ22536 (an adenylate cyclase inhibitor) and ZM241385 (a potent A(2A) receptor antagonist) attenuated the antiplatelet effect of chlorogenic acid. Chlorogenic acid is compatible to the active site of the adenosine A(2A) receptor as revealed through molecular modeling. In addition, chlorogenic acid had a significantly lower effect on mouse bleeding time when compared to the same dose of aspirin. CONCLUSIONS: Antiplatelet and antithrombotic effects of chlorogenic acid are associated with the A(2A) receptor/adenylate cyclase/cAMP/PKA signaling pathway.