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High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks
Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains....
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944860/ https://www.ncbi.nlm.nih.gov/pubmed/24565502 http://dx.doi.org/10.3201/eid2003.131106 |
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author | Tettelin, Hervé Davidson, Rebecca M. Agrawal, Sonia Aitken, Moira L. Shallom, Shamira Hasan, Nabeeh A. Strong, Michael Nogueira de Moura, Vinicius Calado De Groote, Mary Ann Duarte, Rafael S. Hine, Erin Parankush, Sushma Su, Qi Daugherty, Sean C. Fraser, Claire M. Brown-Elliott, Barbara A. Wallace, Richard J. Holland, Steven M. Sampaio, Elizabeth P. Olivier, Kenneth N. Jackson, Mary Zelazny, Adrian M. |
author_facet | Tettelin, Hervé Davidson, Rebecca M. Agrawal, Sonia Aitken, Moira L. Shallom, Shamira Hasan, Nabeeh A. Strong, Michael Nogueira de Moura, Vinicius Calado De Groote, Mary Ann Duarte, Rafael S. Hine, Erin Parankush, Sushma Su, Qi Daugherty, Sean C. Fraser, Claire M. Brown-Elliott, Barbara A. Wallace, Richard J. Holland, Steven M. Sampaio, Elizabeth P. Olivier, Kenneth N. Jackson, Mary Zelazny, Adrian M. |
author_sort | Tettelin, Hervé |
collection | PubMed |
description | Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol. |
format | Online Article Text |
id | pubmed-3944860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-39448602014-03-12 High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks Tettelin, Hervé Davidson, Rebecca M. Agrawal, Sonia Aitken, Moira L. Shallom, Shamira Hasan, Nabeeh A. Strong, Michael Nogueira de Moura, Vinicius Calado De Groote, Mary Ann Duarte, Rafael S. Hine, Erin Parankush, Sushma Su, Qi Daugherty, Sean C. Fraser, Claire M. Brown-Elliott, Barbara A. Wallace, Richard J. Holland, Steven M. Sampaio, Elizabeth P. Olivier, Kenneth N. Jackson, Mary Zelazny, Adrian M. Emerg Infect Dis Research Three recently sequenced strains isolated from patients during an outbreak of Mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the United States were compared with 6 strains from an outbreak at a cystic fibrosis center in the United Kingdom and worldwide strains. Strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in Brazil. We identified unique single-nucleotide polymorphisms in cystic fibrosis and soft tissue outbreak strains, separate single-nucleotide polymorphisms only in cystic fibrosis outbreak strains, and unique genomic traits for each subset of isolates. Our findings highlight the necessity of identifying M. abscessus to the subspecies level and screening all cystic fibrosis isolates for relatedness to these outbreak strains. We propose 2 diagnostic strategies that use partial sequencing of rpoB and secA1 genes and a multilocus sequence typing protocol. Centers for Disease Control and Prevention 2014-03 /pmc/articles/PMC3944860/ /pubmed/24565502 http://dx.doi.org/10.3201/eid2003.131106 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Tettelin, Hervé Davidson, Rebecca M. Agrawal, Sonia Aitken, Moira L. Shallom, Shamira Hasan, Nabeeh A. Strong, Michael Nogueira de Moura, Vinicius Calado De Groote, Mary Ann Duarte, Rafael S. Hine, Erin Parankush, Sushma Su, Qi Daugherty, Sean C. Fraser, Claire M. Brown-Elliott, Barbara A. Wallace, Richard J. Holland, Steven M. Sampaio, Elizabeth P. Olivier, Kenneth N. Jackson, Mary Zelazny, Adrian M. High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks |
title | High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks |
title_full | High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks |
title_fullStr | High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks |
title_full_unstemmed | High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks |
title_short | High-level Relatedness among Mycobacterium abscessus subsp. massiliense Strains from Widely Separated Outbreaks |
title_sort | high-level relatedness among mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944860/ https://www.ncbi.nlm.nih.gov/pubmed/24565502 http://dx.doi.org/10.3201/eid2003.131106 |
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