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Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA

BACKGROUND: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two othe...

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Autores principales: Carrión, Víctor J, van der Voort, Menno, Arrebola, Eva, Gutiérrez-Barranquero, José A, de Vicente, Antonio, Raaijmakers, Jos M, Cazorla, Francisco M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945005/
https://www.ncbi.nlm.nih.gov/pubmed/24555804
http://dx.doi.org/10.1186/1471-2180-14-46
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author Carrión, Víctor J
van der Voort, Menno
Arrebola, Eva
Gutiérrez-Barranquero, José A
de Vicente, Antonio
Raaijmakers, Jos M
Cazorla, Francisco M
author_facet Carrión, Víctor J
van der Voort, Menno
Arrebola, Eva
Gutiérrez-Barranquero, José A
de Vicente, Antonio
Raaijmakers, Jos M
Cazorla, Francisco M
author_sort Carrión, Víctor J
collection PubMed
description BACKGROUND: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. RESULTS: The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. CONCLUSIONS: From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis.
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spelling pubmed-39450052014-03-07 Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA Carrión, Víctor J van der Voort, Menno Arrebola, Eva Gutiérrez-Barranquero, José A de Vicente, Antonio Raaijmakers, Jos M Cazorla, Francisco M BMC Microbiol Research Article BACKGROUND: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. RESULTS: The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. CONCLUSIONS: From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis. BioMed Central 2014-02-21 /pmc/articles/PMC3945005/ /pubmed/24555804 http://dx.doi.org/10.1186/1471-2180-14-46 Text en Copyright © 2014 Carrión et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Carrión, Víctor J
van der Voort, Menno
Arrebola, Eva
Gutiérrez-Barranquero, José A
de Vicente, Antonio
Raaijmakers, Jos M
Cazorla, Francisco M
Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA
title Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA
title_full Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA
title_fullStr Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA
title_full_unstemmed Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA
title_short Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA
title_sort mangotoxin production of pseudomonas syringae pv. syringae is regulated by mgoa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945005/
https://www.ncbi.nlm.nih.gov/pubmed/24555804
http://dx.doi.org/10.1186/1471-2180-14-46
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