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Genetic Basis of Metabolome Variation in Yeast
Metabolism, the conversion of nutrients into usable energy and biochemical building blocks, is an essential feature of all cells. The genetic factors responsible for inter-individual metabolic variability remain poorly understood. To investigate genetic causes of metabolome variation, we measured th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945093/ https://www.ncbi.nlm.nih.gov/pubmed/24603560 http://dx.doi.org/10.1371/journal.pgen.1004142 |
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author | Breunig, Jeffrey S. Hackett, Sean R. Rabinowitz, Joshua D. Kruglyak, Leonid |
author_facet | Breunig, Jeffrey S. Hackett, Sean R. Rabinowitz, Joshua D. Kruglyak, Leonid |
author_sort | Breunig, Jeffrey S. |
collection | PubMed |
description | Metabolism, the conversion of nutrients into usable energy and biochemical building blocks, is an essential feature of all cells. The genetic factors responsible for inter-individual metabolic variability remain poorly understood. To investigate genetic causes of metabolome variation, we measured the concentrations of 74 metabolites across [Image: see text] 100 segregants from a Saccharomyces cerevisiae cross by liquid chromatography-tandem mass spectrometry. We found 52 quantitative trait loci for 34 metabolites. These included linkages due to overt changes in metabolic genes, e.g., linking pyrimidine intermediates to the deletion of ura3. They also included linkages not directly related to metabolic enzymes, such as those for five central carbon metabolites to ira2, a Ras/PKA pathway regulator, and for the metabolites, S-adenosyl-methionine and S-adenosyl-homocysteine to slt2, a MAP kinase involved in cell wall integrity. The variant of ira2 that elevates metabolite levels also increases glucose uptake and ethanol secretion. These results highlight specific examples of genetic variability, including in genes without prior known metabolic regulatory function, that impact yeast metabolism. |
format | Online Article Text |
id | pubmed-3945093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39450932014-03-12 Genetic Basis of Metabolome Variation in Yeast Breunig, Jeffrey S. Hackett, Sean R. Rabinowitz, Joshua D. Kruglyak, Leonid PLoS Genet Research Article Metabolism, the conversion of nutrients into usable energy and biochemical building blocks, is an essential feature of all cells. The genetic factors responsible for inter-individual metabolic variability remain poorly understood. To investigate genetic causes of metabolome variation, we measured the concentrations of 74 metabolites across [Image: see text] 100 segregants from a Saccharomyces cerevisiae cross by liquid chromatography-tandem mass spectrometry. We found 52 quantitative trait loci for 34 metabolites. These included linkages due to overt changes in metabolic genes, e.g., linking pyrimidine intermediates to the deletion of ura3. They also included linkages not directly related to metabolic enzymes, such as those for five central carbon metabolites to ira2, a Ras/PKA pathway regulator, and for the metabolites, S-adenosyl-methionine and S-adenosyl-homocysteine to slt2, a MAP kinase involved in cell wall integrity. The variant of ira2 that elevates metabolite levels also increases glucose uptake and ethanol secretion. These results highlight specific examples of genetic variability, including in genes without prior known metabolic regulatory function, that impact yeast metabolism. Public Library of Science 2014-03-06 /pmc/articles/PMC3945093/ /pubmed/24603560 http://dx.doi.org/10.1371/journal.pgen.1004142 Text en © 2014 Breunig et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Breunig, Jeffrey S. Hackett, Sean R. Rabinowitz, Joshua D. Kruglyak, Leonid Genetic Basis of Metabolome Variation in Yeast |
title | Genetic Basis of Metabolome Variation in Yeast |
title_full | Genetic Basis of Metabolome Variation in Yeast |
title_fullStr | Genetic Basis of Metabolome Variation in Yeast |
title_full_unstemmed | Genetic Basis of Metabolome Variation in Yeast |
title_short | Genetic Basis of Metabolome Variation in Yeast |
title_sort | genetic basis of metabolome variation in yeast |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945093/ https://www.ncbi.nlm.nih.gov/pubmed/24603560 http://dx.doi.org/10.1371/journal.pgen.1004142 |
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