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Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography

The spread of Plasmodium falciparum resistance toward most of the used drugs requires new antimalarial compounds. Taking advantage of the biodiversity, the ethnopharmacological approach opens the way for the discovery and the characterization of potent original molecules. Previous works led to the s...

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Autores principales: Desgrouas, Camille, Desbordes, Marc, Dormoi, Jérôme, Ollivier, Evelyne, Parzy, Daniel, Taudon, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945228/
https://www.ncbi.nlm.nih.gov/pubmed/24693462
http://dx.doi.org/10.1155/2014/695231
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author Desgrouas, Camille
Desbordes, Marc
Dormoi, Jérôme
Ollivier, Evelyne
Parzy, Daniel
Taudon, Nicolas
author_facet Desgrouas, Camille
Desbordes, Marc
Dormoi, Jérôme
Ollivier, Evelyne
Parzy, Daniel
Taudon, Nicolas
author_sort Desgrouas, Camille
collection PubMed
description The spread of Plasmodium falciparum resistance toward most of the used drugs requires new antimalarial compounds. Taking advantage of the biodiversity, the ethnopharmacological approach opens the way for the discovery and the characterization of potent original molecules. Previous works led to the selection of a bisbenzylisoquinoline, cepharanthine, extracted from Stephania rotunda, which is mainly present in Cambodia. A sensitive and selective liquid chromatography method has been developed for the determination of cepharanthine in mouse plasma. The method involved a semiautomated microextraction by packed sorbent (MEPS) using 4 mg of solid phase silica-C8 sorbent. LC separation was performed on a Kinetex XB-C18 column (2.6 µm) with a mobile phase of acetonitrile containing formic acid and 10 mM ammonium formate buffer pH 3.5. Data were acquired at 282 nm with a diode array detector. The drug/internal standard peak area ratios were linked via linear relationships to plasma concentrations (75–2,000 ng/mL). Precision was below 5% and accuracy was 99.0–102%. Extraction recovery of cepharanthine was 56–58%. The method was successfully used to determine the pharmacokinetic profile of cepharanthine in healthy and Plasmodium berghei infected mice. The infection did not impact pharmacokinetic parameters of cepharanthine.
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spelling pubmed-39452282014-04-01 Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography Desgrouas, Camille Desbordes, Marc Dormoi, Jérôme Ollivier, Evelyne Parzy, Daniel Taudon, Nicolas J Anal Methods Chem Research Article The spread of Plasmodium falciparum resistance toward most of the used drugs requires new antimalarial compounds. Taking advantage of the biodiversity, the ethnopharmacological approach opens the way for the discovery and the characterization of potent original molecules. Previous works led to the selection of a bisbenzylisoquinoline, cepharanthine, extracted from Stephania rotunda, which is mainly present in Cambodia. A sensitive and selective liquid chromatography method has been developed for the determination of cepharanthine in mouse plasma. The method involved a semiautomated microextraction by packed sorbent (MEPS) using 4 mg of solid phase silica-C8 sorbent. LC separation was performed on a Kinetex XB-C18 column (2.6 µm) with a mobile phase of acetonitrile containing formic acid and 10 mM ammonium formate buffer pH 3.5. Data were acquired at 282 nm with a diode array detector. The drug/internal standard peak area ratios were linked via linear relationships to plasma concentrations (75–2,000 ng/mL). Precision was below 5% and accuracy was 99.0–102%. Extraction recovery of cepharanthine was 56–58%. The method was successfully used to determine the pharmacokinetic profile of cepharanthine in healthy and Plasmodium berghei infected mice. The infection did not impact pharmacokinetic parameters of cepharanthine. Hindawi Publishing Corporation 2014 2014-02-16 /pmc/articles/PMC3945228/ /pubmed/24693462 http://dx.doi.org/10.1155/2014/695231 Text en Copyright © 2014 Camille Desgrouas et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Desgrouas, Camille
Desbordes, Marc
Dormoi, Jérôme
Ollivier, Evelyne
Parzy, Daniel
Taudon, Nicolas
Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography
title Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography
title_full Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography
title_fullStr Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography
title_full_unstemmed Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography
title_short Quantitative Analysis of Cepharanthine in Plasma Based on Semiautomatic Microextraction by Packed Sorbent Combined with Liquid Chromatography
title_sort quantitative analysis of cepharanthine in plasma based on semiautomatic microextraction by packed sorbent combined with liquid chromatography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945228/
https://www.ncbi.nlm.nih.gov/pubmed/24693462
http://dx.doi.org/10.1155/2014/695231
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