Cargando…

Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections

In this study, phenotypic and genotypic methods were used to detect metallo-β-lactamases, cephalosporinases and oxacillinases and to assess genetic diversity among 64 multiresistant Acinetobacter baumannii strains recovered from blood cultures in five different hospitals in Brazil from December 2008...

Descripción completa

Detalles Bibliográficos
Autores principales: Martins, Hanoch S. I., Bomfim, Maria Rosa Q., França, Rafaela O., Farias, Luiz M., Carvalho, Maria Auxiliadora R., Serufo, José Carlos, Santos, Simone G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945548/
https://www.ncbi.nlm.nih.gov/pubmed/24477210
http://dx.doi.org/10.3390/ijerph110201465
_version_ 1782306538045571072
author Martins, Hanoch S. I.
Bomfim, Maria Rosa Q.
França, Rafaela O.
Farias, Luiz M.
Carvalho, Maria Auxiliadora R.
Serufo, José Carlos
Santos, Simone G.
author_facet Martins, Hanoch S. I.
Bomfim, Maria Rosa Q.
França, Rafaela O.
Farias, Luiz M.
Carvalho, Maria Auxiliadora R.
Serufo, José Carlos
Santos, Simone G.
author_sort Martins, Hanoch S. I.
collection PubMed
description In this study, phenotypic and genotypic methods were used to detect metallo-β-lactamases, cephalosporinases and oxacillinases and to assess genetic diversity among 64 multiresistant Acinetobacter baumannii strains recovered from blood cultures in five different hospitals in Brazil from December 2008 to June 2009. High rates of resistance to imipenem (93.75%) and polymyxin B (39.06%) were observed using the disk diffusion (DD) method and by determining the minimum inhibitory concentration (MIC). Using the disk approximation method, thirty-nine strains (60.9%) were phenotypically positive for class D enzymes, and 51 strains (79.6%) were positive for cephalosporinase (AmpC). Using the E-test, 60 strains (93.75%) were positive for metallo-β-lactamases (MβLs). All strains were positive for at least one of the 10 studied genes; 59 (92.1%) contained bla(VIM-1), 79.6% contained bla(AmpC), 93.7% contained bla(OXA23) and 84.3% contained bla(OXA51). Enterobacteria Repetitive Intergenic Consensus (ERIC)-PCR analysis revealed a predominance of certain clones that differed from each other. However, the same band pattern was observed in samples from the different hospitals studied, demonstrating correlation between the genotypic and phenotypic results. Thus, ERIC-PCR is an appropriate method for rapidly clustering genetically related isolates. These results suggest that defined clonal clusters are circulating within the studied hospitals. These results also show that the prevalence of MDR A. baumannii may vary among clones disseminated in specific hospitals, and they emphasize the importance of adhering to appropriate infection control measures.
format Online
Article
Text
id pubmed-3945548
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-39455482014-03-10 Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections Martins, Hanoch S. I. Bomfim, Maria Rosa Q. França, Rafaela O. Farias, Luiz M. Carvalho, Maria Auxiliadora R. Serufo, José Carlos Santos, Simone G. Int J Environ Res Public Health In this study, phenotypic and genotypic methods were used to detect metallo-β-lactamases, cephalosporinases and oxacillinases and to assess genetic diversity among 64 multiresistant Acinetobacter baumannii strains recovered from blood cultures in five different hospitals in Brazil from December 2008 to June 2009. High rates of resistance to imipenem (93.75%) and polymyxin B (39.06%) were observed using the disk diffusion (DD) method and by determining the minimum inhibitory concentration (MIC). Using the disk approximation method, thirty-nine strains (60.9%) were phenotypically positive for class D enzymes, and 51 strains (79.6%) were positive for cephalosporinase (AmpC). Using the E-test, 60 strains (93.75%) were positive for metallo-β-lactamases (MβLs). All strains were positive for at least one of the 10 studied genes; 59 (92.1%) contained bla(VIM-1), 79.6% contained bla(AmpC), 93.7% contained bla(OXA23) and 84.3% contained bla(OXA51). Enterobacteria Repetitive Intergenic Consensus (ERIC)-PCR analysis revealed a predominance of certain clones that differed from each other. However, the same band pattern was observed in samples from the different hospitals studied, demonstrating correlation between the genotypic and phenotypic results. Thus, ERIC-PCR is an appropriate method for rapidly clustering genetically related isolates. These results suggest that defined clonal clusters are circulating within the studied hospitals. These results also show that the prevalence of MDR A. baumannii may vary among clones disseminated in specific hospitals, and they emphasize the importance of adhering to appropriate infection control measures. MDPI 2014-01-28 2014-02 /pmc/articles/PMC3945548/ /pubmed/24477210 http://dx.doi.org/10.3390/ijerph110201465 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Martins, Hanoch S. I.
Bomfim, Maria Rosa Q.
França, Rafaela O.
Farias, Luiz M.
Carvalho, Maria Auxiliadora R.
Serufo, José Carlos
Santos, Simone G.
Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections
title Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections
title_full Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections
title_fullStr Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections
title_full_unstemmed Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections
title_short Resistance Markers and Genetic Diversity in Acinetobacter baumannii Strains Recovered from Nosocomial Bloodstream Infections
title_sort resistance markers and genetic diversity in acinetobacter baumannii strains recovered from nosocomial bloodstream infections
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945548/
https://www.ncbi.nlm.nih.gov/pubmed/24477210
http://dx.doi.org/10.3390/ijerph110201465
work_keys_str_mv AT martinshanochsi resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections
AT bomfimmariarosaq resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections
AT francarafaelao resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections
AT fariasluizm resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections
AT carvalhomariaauxiliadorar resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections
AT serufojosecarlos resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections
AT santossimoneg resistancemarkersandgeneticdiversityinacinetobacterbaumanniistrainsrecoveredfromnosocomialbloodstreaminfections