Cargando…

Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy

T-type Ca(2+) channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a sl...

Descripción completa

Detalles Bibliográficos
Autores principales: Khomula, Eugen V., Borisyuk, Anya L., Viatchenko-Karpinski, Viacheslav Y., Briede, Andrea, Belan, Pavel V., Voitenko, Nana V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945737/
https://www.ncbi.nlm.nih.gov/pubmed/24693454
http://dx.doi.org/10.1155/2014/938235
_version_ 1782306565562302464
author Khomula, Eugen V.
Borisyuk, Anya L.
Viatchenko-Karpinski, Viacheslav Y.
Briede, Andrea
Belan, Pavel V.
Voitenko, Nana V.
author_facet Khomula, Eugen V.
Borisyuk, Anya L.
Viatchenko-Karpinski, Viacheslav Y.
Briede, Andrea
Belan, Pavel V.
Voitenko, Nana V.
author_sort Khomula, Eugen V.
collection PubMed
description T-type Ca(2+) channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow T-type Ca(2+) current (T-current) while a fast T-current is expressed in the other 70% of these neurons. Streptozotocin-induced diabetes in young rats resulted in thermal hyperalgesia, hypoalgesia, or normalgesia 5-6 weeks after the induction. Our results show that NTCN neurons obtained from hyperalgesic animals do not express the slow T-current. Meanwhile, the fraction of neurons expressing the slow T-current did not significantly change in the hypo- and normalgesic diabetic groups. Moreover, the peak current density of fast T-current was significantly increased only in the neurons of hyperalgesic group. In contrast, the peak current density of slow T-current was significantly decreased in the hypo- and normalgesic groups. Experimental diabetes also resulted in a depolarizing shift of steady-state inactivation of fast T-current in the hyperalgesic group and slow T-current in the hypo- and normalgesic groups. We suggest that the observed changes may contribute to expression of different types of peripheral diabetic neuropathy occurring during the development of diabetes mellitus.
format Online
Article
Text
id pubmed-3945737
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-39457372014-04-01 Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy Khomula, Eugen V. Borisyuk, Anya L. Viatchenko-Karpinski, Viacheslav Y. Briede, Andrea Belan, Pavel V. Voitenko, Nana V. Neural Plast Research Article T-type Ca(2+) channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow T-type Ca(2+) current (T-current) while a fast T-current is expressed in the other 70% of these neurons. Streptozotocin-induced diabetes in young rats resulted in thermal hyperalgesia, hypoalgesia, or normalgesia 5-6 weeks after the induction. Our results show that NTCN neurons obtained from hyperalgesic animals do not express the slow T-current. Meanwhile, the fraction of neurons expressing the slow T-current did not significantly change in the hypo- and normalgesic diabetic groups. Moreover, the peak current density of fast T-current was significantly increased only in the neurons of hyperalgesic group. In contrast, the peak current density of slow T-current was significantly decreased in the hypo- and normalgesic groups. Experimental diabetes also resulted in a depolarizing shift of steady-state inactivation of fast T-current in the hyperalgesic group and slow T-current in the hypo- and normalgesic groups. We suggest that the observed changes may contribute to expression of different types of peripheral diabetic neuropathy occurring during the development of diabetes mellitus. Hindawi Publishing Corporation 2014 2014-02-18 /pmc/articles/PMC3945737/ /pubmed/24693454 http://dx.doi.org/10.1155/2014/938235 Text en Copyright © 2014 Eugen V. Khomula et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Khomula, Eugen V.
Borisyuk, Anya L.
Viatchenko-Karpinski, Viacheslav Y.
Briede, Andrea
Belan, Pavel V.
Voitenko, Nana V.
Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
title Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
title_full Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
title_fullStr Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
title_full_unstemmed Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
title_short Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
title_sort nociceptive neurons differentially express fast and slow t-type ca(2+) currents in different types of diabetic neuropathy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945737/
https://www.ncbi.nlm.nih.gov/pubmed/24693454
http://dx.doi.org/10.1155/2014/938235
work_keys_str_mv AT khomulaeugenv nociceptiveneuronsdifferentiallyexpressfastandslowttypeca2currentsindifferenttypesofdiabeticneuropathy
AT borisyukanyal nociceptiveneuronsdifferentiallyexpressfastandslowttypeca2currentsindifferenttypesofdiabeticneuropathy
AT viatchenkokarpinskiviacheslavy nociceptiveneuronsdifferentiallyexpressfastandslowttypeca2currentsindifferenttypesofdiabeticneuropathy
AT briedeandrea nociceptiveneuronsdifferentiallyexpressfastandslowttypeca2currentsindifferenttypesofdiabeticneuropathy
AT belanpavelv nociceptiveneuronsdifferentiallyexpressfastandslowttypeca2currentsindifferenttypesofdiabeticneuropathy
AT voitenkonanav nociceptiveneuronsdifferentiallyexpressfastandslowttypeca2currentsindifferenttypesofdiabeticneuropathy