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Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy
T-type Ca(2+) channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a sl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945737/ https://www.ncbi.nlm.nih.gov/pubmed/24693454 http://dx.doi.org/10.1155/2014/938235 |
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author | Khomula, Eugen V. Borisyuk, Anya L. Viatchenko-Karpinski, Viacheslav Y. Briede, Andrea Belan, Pavel V. Voitenko, Nana V. |
author_facet | Khomula, Eugen V. Borisyuk, Anya L. Viatchenko-Karpinski, Viacheslav Y. Briede, Andrea Belan, Pavel V. Voitenko, Nana V. |
author_sort | Khomula, Eugen V. |
collection | PubMed |
description | T-type Ca(2+) channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow T-type Ca(2+) current (T-current) while a fast T-current is expressed in the other 70% of these neurons. Streptozotocin-induced diabetes in young rats resulted in thermal hyperalgesia, hypoalgesia, or normalgesia 5-6 weeks after the induction. Our results show that NTCN neurons obtained from hyperalgesic animals do not express the slow T-current. Meanwhile, the fraction of neurons expressing the slow T-current did not significantly change in the hypo- and normalgesic diabetic groups. Moreover, the peak current density of fast T-current was significantly increased only in the neurons of hyperalgesic group. In contrast, the peak current density of slow T-current was significantly decreased in the hypo- and normalgesic groups. Experimental diabetes also resulted in a depolarizing shift of steady-state inactivation of fast T-current in the hyperalgesic group and slow T-current in the hypo- and normalgesic groups. We suggest that the observed changes may contribute to expression of different types of peripheral diabetic neuropathy occurring during the development of diabetes mellitus. |
format | Online Article Text |
id | pubmed-3945737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39457372014-04-01 Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy Khomula, Eugen V. Borisyuk, Anya L. Viatchenko-Karpinski, Viacheslav Y. Briede, Andrea Belan, Pavel V. Voitenko, Nana V. Neural Plast Research Article T-type Ca(2+) channels are known as important participants of nociception and their remodeling contributes to diabetes-induced alterations of pain sensation. In this work we have established that about 30% of rat nonpeptidergic thermal C-type nociceptive (NTCN) neurons of segments L4–L6 express a slow T-type Ca(2+) current (T-current) while a fast T-current is expressed in the other 70% of these neurons. Streptozotocin-induced diabetes in young rats resulted in thermal hyperalgesia, hypoalgesia, or normalgesia 5-6 weeks after the induction. Our results show that NTCN neurons obtained from hyperalgesic animals do not express the slow T-current. Meanwhile, the fraction of neurons expressing the slow T-current did not significantly change in the hypo- and normalgesic diabetic groups. Moreover, the peak current density of fast T-current was significantly increased only in the neurons of hyperalgesic group. In contrast, the peak current density of slow T-current was significantly decreased in the hypo- and normalgesic groups. Experimental diabetes also resulted in a depolarizing shift of steady-state inactivation of fast T-current in the hyperalgesic group and slow T-current in the hypo- and normalgesic groups. We suggest that the observed changes may contribute to expression of different types of peripheral diabetic neuropathy occurring during the development of diabetes mellitus. Hindawi Publishing Corporation 2014 2014-02-18 /pmc/articles/PMC3945737/ /pubmed/24693454 http://dx.doi.org/10.1155/2014/938235 Text en Copyright © 2014 Eugen V. Khomula et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Khomula, Eugen V. Borisyuk, Anya L. Viatchenko-Karpinski, Viacheslav Y. Briede, Andrea Belan, Pavel V. Voitenko, Nana V. Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy |
title | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy |
title_full | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy |
title_fullStr | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy |
title_full_unstemmed | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy |
title_short | Nociceptive Neurons Differentially Express Fast and Slow T-Type Ca(2+) Currents in Different Types of Diabetic Neuropathy |
title_sort | nociceptive neurons differentially express fast and slow t-type ca(2+) currents in different types of diabetic neuropathy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945737/ https://www.ncbi.nlm.nih.gov/pubmed/24693454 http://dx.doi.org/10.1155/2014/938235 |
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