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Bacterial communities on classroom surfaces vary with human contact

BACKGROUND: Humans can spend the majority of their time indoors, but little is known about the interactions between the human and built-environment microbiomes or the forces that drive microbial community assembly in the built environment. We sampled 16S rRNA genes from four different surface types...

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Autores principales: Meadow, James F, Altrichter, Adam E, Kembel, Steven W, Moriyama, Maxwell, O’Connor, Timothy K, Womack, Ann M, Brown, G Z, Green, Jessica L, Bohannan, Brendan J M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945812/
https://www.ncbi.nlm.nih.gov/pubmed/24602274
http://dx.doi.org/10.1186/2049-2618-2-7
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author Meadow, James F
Altrichter, Adam E
Kembel, Steven W
Moriyama, Maxwell
O’Connor, Timothy K
Womack, Ann M
Brown, G Z
Green, Jessica L
Bohannan, Brendan J M
author_facet Meadow, James F
Altrichter, Adam E
Kembel, Steven W
Moriyama, Maxwell
O’Connor, Timothy K
Womack, Ann M
Brown, G Z
Green, Jessica L
Bohannan, Brendan J M
author_sort Meadow, James F
collection PubMed
description BACKGROUND: Humans can spend the majority of their time indoors, but little is known about the interactions between the human and built-environment microbiomes or the forces that drive microbial community assembly in the built environment. We sampled 16S rRNA genes from four different surface types throughout a university classroom to determine whether bacterial assemblages on each surface were best predicted by routine human interactions or by proximity to other surfaces within the classroom. We then analyzed our data with publicly-available datasets representing potential source environments. RESULTS: Bacterial assemblages from the four surface types, as well as individual taxa, were indicative of different source pools related to the type of human contact each surface routinely encounters. Spatial proximity to other surfaces in the classroom did not predict community composition. CONCLUSIONS: Our results indicate that human-associated microbial communities can be transferred to indoor surfaces following contact, and that such transmission is possible even when contact is indirect, but that proximity to other surfaces in the classroom does not influence community composition.
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spelling pubmed-39458122014-03-08 Bacterial communities on classroom surfaces vary with human contact Meadow, James F Altrichter, Adam E Kembel, Steven W Moriyama, Maxwell O’Connor, Timothy K Womack, Ann M Brown, G Z Green, Jessica L Bohannan, Brendan J M Microbiome Research BACKGROUND: Humans can spend the majority of their time indoors, but little is known about the interactions between the human and built-environment microbiomes or the forces that drive microbial community assembly in the built environment. We sampled 16S rRNA genes from four different surface types throughout a university classroom to determine whether bacterial assemblages on each surface were best predicted by routine human interactions or by proximity to other surfaces within the classroom. We then analyzed our data with publicly-available datasets representing potential source environments. RESULTS: Bacterial assemblages from the four surface types, as well as individual taxa, were indicative of different source pools related to the type of human contact each surface routinely encounters. Spatial proximity to other surfaces in the classroom did not predict community composition. CONCLUSIONS: Our results indicate that human-associated microbial communities can be transferred to indoor surfaces following contact, and that such transmission is possible even when contact is indirect, but that proximity to other surfaces in the classroom does not influence community composition. BioMed Central 2014-03-07 /pmc/articles/PMC3945812/ /pubmed/24602274 http://dx.doi.org/10.1186/2049-2618-2-7 Text en Copyright © 2014 Meadow et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Meadow, James F
Altrichter, Adam E
Kembel, Steven W
Moriyama, Maxwell
O’Connor, Timothy K
Womack, Ann M
Brown, G Z
Green, Jessica L
Bohannan, Brendan J M
Bacterial communities on classroom surfaces vary with human contact
title Bacterial communities on classroom surfaces vary with human contact
title_full Bacterial communities on classroom surfaces vary with human contact
title_fullStr Bacterial communities on classroom surfaces vary with human contact
title_full_unstemmed Bacterial communities on classroom surfaces vary with human contact
title_short Bacterial communities on classroom surfaces vary with human contact
title_sort bacterial communities on classroom surfaces vary with human contact
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945812/
https://www.ncbi.nlm.nih.gov/pubmed/24602274
http://dx.doi.org/10.1186/2049-2618-2-7
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