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Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease
BACKGROUND: Cystic fibrosis (CF) lung disease begins in early life and is progressive with the major risk factor being an exaggerated inflammatory response. Currently, assessment of neutrophilic inflammation in early cystic fibrosis (CF) lung disease relies on bronchoalveolar lavage (BAL). The chiti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946043/ https://www.ncbi.nlm.nih.gov/pubmed/24576297 http://dx.doi.org/10.1186/1471-2466-14-28 |
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author | Fantino, Emmanuelle Gangell, Catherine L Hartl, Dominik Sly, Peter D |
author_facet | Fantino, Emmanuelle Gangell, Catherine L Hartl, Dominik Sly, Peter D |
author_sort | Fantino, Emmanuelle |
collection | PubMed |
description | BACKGROUND: Cystic fibrosis (CF) lung disease begins in early life and is progressive with the major risk factor being an exaggerated inflammatory response. Currently, assessment of neutrophilic inflammation in early cystic fibrosis (CF) lung disease relies on bronchoalveolar lavage (BAL). The chitinase-like protein YKL-40 is raised in sputum and serum of adults with CF. We investigated YKL-40 in BAL, serum and urine to determine whether this reflected inflammation and infection in young children with CF. METHODS: YKL-40 was measured in matched samples of BAL, serum and urine obtained from 36 infants and young children with CF participating in an early surveillance program. Levels were compared to clinical data and markers of inflammation detected in the lung. RESULTS: YKL-40 in BAL correlated with pulmonary infection [β=1.30 (SE 0.34), p < 0.001] and BAL markers of inflammation [macrophage number: r(2) = 0.34, p < 0.001; neutrophil number: r(2) = 0.74, p < 0.001; neutrophil elastase: r(2) = 0.47, p < 0.001; CXCL8: r(2) = 0.45, p < 0.001; IL-β: r(2) = 0.62, p < 0.001]. YKL-40 was detectable in serum but levels did not correlate with BAL levels in the same individuals (r(2) = 0.04, p = 0.14) or with inflammatory markers. YKL-40 was below the limit of detection in urine (30 pg/ml). CONCLUSIONS: This study demonstrates that levels of the chitinase-like protein YKL-40 reflect airway inflammation and infection in early CF lung disease. The lack of increased YKL-40 in serum in the absence of systemic inflammation limits the benefit of this potential biomarker in early disease. |
format | Online Article Text |
id | pubmed-3946043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39460432014-03-09 Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease Fantino, Emmanuelle Gangell, Catherine L Hartl, Dominik Sly, Peter D BMC Pulm Med Research Article BACKGROUND: Cystic fibrosis (CF) lung disease begins in early life and is progressive with the major risk factor being an exaggerated inflammatory response. Currently, assessment of neutrophilic inflammation in early cystic fibrosis (CF) lung disease relies on bronchoalveolar lavage (BAL). The chitinase-like protein YKL-40 is raised in sputum and serum of adults with CF. We investigated YKL-40 in BAL, serum and urine to determine whether this reflected inflammation and infection in young children with CF. METHODS: YKL-40 was measured in matched samples of BAL, serum and urine obtained from 36 infants and young children with CF participating in an early surveillance program. Levels were compared to clinical data and markers of inflammation detected in the lung. RESULTS: YKL-40 in BAL correlated with pulmonary infection [β=1.30 (SE 0.34), p < 0.001] and BAL markers of inflammation [macrophage number: r(2) = 0.34, p < 0.001; neutrophil number: r(2) = 0.74, p < 0.001; neutrophil elastase: r(2) = 0.47, p < 0.001; CXCL8: r(2) = 0.45, p < 0.001; IL-β: r(2) = 0.62, p < 0.001]. YKL-40 was detectable in serum but levels did not correlate with BAL levels in the same individuals (r(2) = 0.04, p = 0.14) or with inflammatory markers. YKL-40 was below the limit of detection in urine (30 pg/ml). CONCLUSIONS: This study demonstrates that levels of the chitinase-like protein YKL-40 reflect airway inflammation and infection in early CF lung disease. The lack of increased YKL-40 in serum in the absence of systemic inflammation limits the benefit of this potential biomarker in early disease. BioMed Central 2014-02-27 /pmc/articles/PMC3946043/ /pubmed/24576297 http://dx.doi.org/10.1186/1471-2466-14-28 Text en Copyright © 2014 Fantino et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Fantino, Emmanuelle Gangell, Catherine L Hartl, Dominik Sly, Peter D Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease |
title | Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease |
title_full | Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease |
title_fullStr | Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease |
title_full_unstemmed | Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease |
title_short | Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease |
title_sort | airway, but not serum or urinary, levels of ykl-40 reflect inflammation in early cystic fibrosis lung disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946043/ https://www.ncbi.nlm.nih.gov/pubmed/24576297 http://dx.doi.org/10.1186/1471-2466-14-28 |
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