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Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major

INTRODUCTION: To determine the immunostimulatory potential of crude extracts of Warburgia ugandensis subsp. ugandensis with a soluble leishmanial antigen in vaccinating BALB/c mice. METHODS: Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble...

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Autores principales: Ngure, Peter, Ng'ang'a, Zipporah, Kimutai, Albert, Kepha, Stella, Mong'are, Samuel, Ingonga, Johnnie, Tonui, Willy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946221/
https://www.ncbi.nlm.nih.gov/pubmed/24624248
http://dx.doi.org/10.11694/pamj.supp.2014.17.1.3638
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author Ngure, Peter
Ng'ang'a, Zipporah
Kimutai, Albert
Kepha, Stella
Mong'are, Samuel
Ingonga, Johnnie
Tonui, Willy
author_facet Ngure, Peter
Ng'ang'a, Zipporah
Kimutai, Albert
Kepha, Stella
Mong'are, Samuel
Ingonga, Johnnie
Tonui, Willy
author_sort Ngure, Peter
collection PubMed
description INTRODUCTION: To determine the immunostimulatory potential of crude extracts of Warburgia ugandensis subsp. ugandensis with a soluble leishmanial antigen in vaccinating BALB/c mice. METHODS: Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble leishmania antigens (SLA) alone, hexane, ethyl acetate, and dichloromethane extract co-administered with SLA. Unvaccinated mice formed the control group. The induction of cell-mediated immunity following vaccination was determined by measuring in vitro lymphocyte proliferation and the production of interleukin (IL)-4 and gamma interferon (IFN-γ) determined by flow cytometry. Protection against L. major was determined by quantifying parasite burdens in L. major infected footpads using a limiting dilution assay and by measuring lesion sizes of the infected footpad compared to the contralateral uninfected footpad. RESULTS: On vaccination with extracts of W. ugandensis subsp. ugandensis alone or as adjuvants when used in combination with Leishmania antigens, the hexane extract and the dichloromethane extract plus SLA stimulated moderate production of IFN-γ and low levels of IL-4.These mice were partially protected from cutaneous leishmaniasis as shown by the slow development of lesions and comparatively less parasite burdens. CONCLUSION: These data suggest that extracts of W. ugandensis subsp. ugandensis are suitable adjuvants for Leishmania vaccines. However, since W. ugandensis subsp. ugandensis has been shown to be effective against Leishmania parasites in vitro and in vivo, further studies ought to be conducted to determine its immunochemotherapeutic potential when co-administered with a soluble leishmanial antigen in vaccinating BALB/c mice.
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spelling pubmed-39462212014-03-12 Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major Ngure, Peter Ng'ang'a, Zipporah Kimutai, Albert Kepha, Stella Mong'are, Samuel Ingonga, Johnnie Tonui, Willy Pan Afr Med J Research INTRODUCTION: To determine the immunostimulatory potential of crude extracts of Warburgia ugandensis subsp. ugandensis with a soluble leishmanial antigen in vaccinating BALB/c mice. METHODS: Seventy two female BALB/c mice were randomly assigned into six groups. The mice were vaccinated with soluble leishmania antigens (SLA) alone, hexane, ethyl acetate, and dichloromethane extract co-administered with SLA. Unvaccinated mice formed the control group. The induction of cell-mediated immunity following vaccination was determined by measuring in vitro lymphocyte proliferation and the production of interleukin (IL)-4 and gamma interferon (IFN-γ) determined by flow cytometry. Protection against L. major was determined by quantifying parasite burdens in L. major infected footpads using a limiting dilution assay and by measuring lesion sizes of the infected footpad compared to the contralateral uninfected footpad. RESULTS: On vaccination with extracts of W. ugandensis subsp. ugandensis alone or as adjuvants when used in combination with Leishmania antigens, the hexane extract and the dichloromethane extract plus SLA stimulated moderate production of IFN-γ and low levels of IL-4.These mice were partially protected from cutaneous leishmaniasis as shown by the slow development of lesions and comparatively less parasite burdens. CONCLUSION: These data suggest that extracts of W. ugandensis subsp. ugandensis are suitable adjuvants for Leishmania vaccines. However, since W. ugandensis subsp. ugandensis has been shown to be effective against Leishmania parasites in vitro and in vivo, further studies ought to be conducted to determine its immunochemotherapeutic potential when co-administered with a soluble leishmanial antigen in vaccinating BALB/c mice. The African Field Epidemiology Network 2014-01-18 /pmc/articles/PMC3946221/ /pubmed/24624248 http://dx.doi.org/10.11694/pamj.supp.2014.17.1.3638 Text en © Peter Ngure et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ngure, Peter
Ng'ang'a, Zipporah
Kimutai, Albert
Kepha, Stella
Mong'are, Samuel
Ingonga, Johnnie
Tonui, Willy
Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
title Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
title_full Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
title_fullStr Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
title_full_unstemmed Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
title_short Immunostimulatory responses to crude extracts of Warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by Balb/c mice infected with Leishmania major
title_sort immunostimulatory responses to crude extracts of warburgia ugandensis (sprague) subsp ugandensis (canellaceae) by balb/c mice infected with leishmania major
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946221/
https://www.ncbi.nlm.nih.gov/pubmed/24624248
http://dx.doi.org/10.11694/pamj.supp.2014.17.1.3638
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