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Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France

In France, the use of the 7-valent pneumococcal conjugate vaccine (PCV7) lead to an overall significant decrease in PCV7 invasive pneumococcal disease (IPD) incidence. However, the decrease in vaccine serotype prevalence was partially counterbalanced by the serotype replacement phenomenon. In this s...

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Autores principales: Janoir, Claire, Cohen, Robert, Levy, Corinne, Bingen, Edouard, Lepoutre, Agnès, Gutmann, Laurent, Varon, Emmanuelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946248/
https://www.ncbi.nlm.nih.gov/pubmed/24603763
http://dx.doi.org/10.1371/journal.pone.0090935
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author Janoir, Claire
Cohen, Robert
Levy, Corinne
Bingen, Edouard
Lepoutre, Agnès
Gutmann, Laurent
Varon, Emmanuelle
author_facet Janoir, Claire
Cohen, Robert
Levy, Corinne
Bingen, Edouard
Lepoutre, Agnès
Gutmann, Laurent
Varon, Emmanuelle
author_sort Janoir, Claire
collection PubMed
description In France, the use of the 7-valent pneumococcal conjugate vaccine (PCV7) lead to an overall significant decrease in PCV7 invasive pneumococcal disease (IPD) incidence. However, the decrease in vaccine serotype prevalence was partially counterbalanced by the serotype replacement phenomenon. In this study, we analyzed the role of the newly described serotype 6C as one of the replacement serotypes. This work was conducted on a large time scale from the early PCV7 era (2002–2003) to the PCV13 era (2010–2011), both on IPD strains recovered from the whole population and nasopharyngeal colonizing strains isolated in infant less than two years, who are known to be the main reservoir for pneumococci. Serotype 6C took advantage over 6A and 6B serotypes, which both decreased over time. A continuous and significant increase in 6C IPD was observed in adults along the study period; in contrast, in children less than two years, only an increase in 6C nasopharyngeal carriage was found, the prevalence of serotype 6C in IPD remaining very low over time. Among 101 6C invasive and colonizing strains studied by MLST, 24 STs were found to be related to three major clonal complexes, CC395, CC176, and CC315. STs related to CC176 tend to disappear after 2009 and were essentially replaced by ST386 (CC315), which dramatically increased over time. This clonal expansion may be explained by the erythromycin and tetracycline resistances associated with this clone. Finally, the decrease observed in nasopharyngeal 6C carriage since 2010, likely related to the PCV13 introduction in the French immunization schedule, is expected to lead to a decrease in 6C IPD in adults thereafter.
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spelling pubmed-39462482014-03-12 Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France Janoir, Claire Cohen, Robert Levy, Corinne Bingen, Edouard Lepoutre, Agnès Gutmann, Laurent Varon, Emmanuelle PLoS One Research Article In France, the use of the 7-valent pneumococcal conjugate vaccine (PCV7) lead to an overall significant decrease in PCV7 invasive pneumococcal disease (IPD) incidence. However, the decrease in vaccine serotype prevalence was partially counterbalanced by the serotype replacement phenomenon. In this study, we analyzed the role of the newly described serotype 6C as one of the replacement serotypes. This work was conducted on a large time scale from the early PCV7 era (2002–2003) to the PCV13 era (2010–2011), both on IPD strains recovered from the whole population and nasopharyngeal colonizing strains isolated in infant less than two years, who are known to be the main reservoir for pneumococci. Serotype 6C took advantage over 6A and 6B serotypes, which both decreased over time. A continuous and significant increase in 6C IPD was observed in adults along the study period; in contrast, in children less than two years, only an increase in 6C nasopharyngeal carriage was found, the prevalence of serotype 6C in IPD remaining very low over time. Among 101 6C invasive and colonizing strains studied by MLST, 24 STs were found to be related to three major clonal complexes, CC395, CC176, and CC315. STs related to CC176 tend to disappear after 2009 and were essentially replaced by ST386 (CC315), which dramatically increased over time. This clonal expansion may be explained by the erythromycin and tetracycline resistances associated with this clone. Finally, the decrease observed in nasopharyngeal 6C carriage since 2010, likely related to the PCV13 introduction in the French immunization schedule, is expected to lead to a decrease in 6C IPD in adults thereafter. Public Library of Science 2014-03-06 /pmc/articles/PMC3946248/ /pubmed/24603763 http://dx.doi.org/10.1371/journal.pone.0090935 Text en © 2014 Janoir et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Janoir, Claire
Cohen, Robert
Levy, Corinne
Bingen, Edouard
Lepoutre, Agnès
Gutmann, Laurent
Varon, Emmanuelle
Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France
title Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France
title_full Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France
title_fullStr Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France
title_full_unstemmed Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France
title_short Clonal Expansion of the Macrolide Resistant ST386 within Pneumococcal Serotype 6C in France
title_sort clonal expansion of the macrolide resistant st386 within pneumococcal serotype 6c in france
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946248/
https://www.ncbi.nlm.nih.gov/pubmed/24603763
http://dx.doi.org/10.1371/journal.pone.0090935
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