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Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia
Berberine is one kind of isoquinoline alkaloid with anti-apoptotic effects on the neurons suffering ischemia. To address the explanation for these activities, the berberine-induced cell cycle arrest during neurons suffering ischemia/reperfusion had been studied in the present study. According to the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946351/ https://www.ncbi.nlm.nih.gov/pubmed/24603897 http://dx.doi.org/10.1371/journal.pone.0090850 |
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author | Chai, Yu-Shuang Yuan, Zhi-Yi Lei, Fan Wang, Yu-Gang Hu, Jun Du, Feng Lu, Xi Jiang, Jing-Fei Xing, Dong-Ming Du, Li-Jun |
author_facet | Chai, Yu-Shuang Yuan, Zhi-Yi Lei, Fan Wang, Yu-Gang Hu, Jun Du, Feng Lu, Xi Jiang, Jing-Fei Xing, Dong-Ming Du, Li-Jun |
author_sort | Chai, Yu-Shuang |
collection | PubMed |
description | Berberine is one kind of isoquinoline alkaloid with anti-apoptotic effects on the neurons suffering ischemia. To address the explanation for these activities, the berberine-induced cell cycle arrest during neurons suffering ischemia/reperfusion had been studied in the present study. According to the in vitro neurons with oxygen-glucose deprivation and in vivo ICR mice with cerebral ischemia/reperfusion, it was found that berberine could protect the mRNA of retinoblastoma (Rb) from degradation through its function on the poly(A) tail. The prolonged half-life of retinoblastoma 1 (gene of Rb, RB1) mRNA level secures the protein level of retinoblastoma, which facilitates cell cycle arrest of neurons in the process of ischemia/reperfusion and subsequently avoids cells entering in the apoptotic process. The poly(A) tail of RB1 mRNA, as a newly identified target of berberine, could help people focus on the interaction between berberine and mRNA to further understand the biological activities and functions of berberine. |
format | Online Article Text |
id | pubmed-3946351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39463512014-03-12 Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia Chai, Yu-Shuang Yuan, Zhi-Yi Lei, Fan Wang, Yu-Gang Hu, Jun Du, Feng Lu, Xi Jiang, Jing-Fei Xing, Dong-Ming Du, Li-Jun PLoS One Research Article Berberine is one kind of isoquinoline alkaloid with anti-apoptotic effects on the neurons suffering ischemia. To address the explanation for these activities, the berberine-induced cell cycle arrest during neurons suffering ischemia/reperfusion had been studied in the present study. According to the in vitro neurons with oxygen-glucose deprivation and in vivo ICR mice with cerebral ischemia/reperfusion, it was found that berberine could protect the mRNA of retinoblastoma (Rb) from degradation through its function on the poly(A) tail. The prolonged half-life of retinoblastoma 1 (gene of Rb, RB1) mRNA level secures the protein level of retinoblastoma, which facilitates cell cycle arrest of neurons in the process of ischemia/reperfusion and subsequently avoids cells entering in the apoptotic process. The poly(A) tail of RB1 mRNA, as a newly identified target of berberine, could help people focus on the interaction between berberine and mRNA to further understand the biological activities and functions of berberine. Public Library of Science 2014-03-06 /pmc/articles/PMC3946351/ /pubmed/24603897 http://dx.doi.org/10.1371/journal.pone.0090850 Text en © 2014 Chai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chai, Yu-Shuang Yuan, Zhi-Yi Lei, Fan Wang, Yu-Gang Hu, Jun Du, Feng Lu, Xi Jiang, Jing-Fei Xing, Dong-Ming Du, Li-Jun Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia |
title | Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia |
title_full | Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia |
title_fullStr | Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia |
title_full_unstemmed | Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia |
title_short | Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia |
title_sort | inhibition of retinoblastoma mrna degradation through poly (a) involved in the neuroprotective effect of berberine against cerebral ischemia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3946351/ https://www.ncbi.nlm.nih.gov/pubmed/24603897 http://dx.doi.org/10.1371/journal.pone.0090850 |
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