H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin

The O(2)-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H(2)O(2)-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol...

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Autores principales: Okamoto, Yasunori, Onoda, Akira, Sugimoto, Hiroshi, Takano, Yu, Hirota, Shun, Kurtz, Donald M., Shiro, Yoshitsugu, Hayashi, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2014
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947700/
https://www.ncbi.nlm.nih.gov/pubmed/24400317
http://dx.doi.org/10.1039/c3cc48108e
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author Okamoto, Yasunori
Onoda, Akira
Sugimoto, Hiroshi
Takano, Yu
Hirota, Shun
Kurtz, Donald M.
Shiro, Yoshitsugu
Hayashi, Takashi
author_facet Okamoto, Yasunori
Onoda, Akira
Sugimoto, Hiroshi
Takano, Yu
Hirota, Shun
Kurtz, Donald M.
Shiro, Yoshitsugu
Hayashi, Takashi
author_sort Okamoto, Yasunori
collection PubMed
description The O(2)-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H(2)O(2)-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H(2)O(2).
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spelling pubmed-39477002014-11-05 H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin Okamoto, Yasunori Onoda, Akira Sugimoto, Hiroshi Takano, Yu Hirota, Shun Kurtz, Donald M. Shiro, Yoshitsugu Hayashi, Takashi Chem Commun (Camb) Chemistry The O(2)-binding carboxylate-bridged diiron site in DcrH-Hr was engineered in an effort to perform the H(2)O(2)-dependent oxidation of external substrates. A His residue was introduced near the diiron site in place of a conserved residue, Ile119. The I119H variant promotes the oxidation of guaiacol and 1,4-cyclohexadiene upon addition of H(2)O(2). Royal Society of Chemistry 2014-04-04 2014-01-08 /pmc/articles/PMC3947700/ /pubmed/24400317 http://dx.doi.org/10.1039/c3cc48108e Text en This journal is © The Royal Society of Chemistry 2014 https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Okamoto, Yasunori
Onoda, Akira
Sugimoto, Hiroshi
Takano, Yu
Hirota, Shun
Kurtz, Donald M.
Shiro, Yoshitsugu
Hayashi, Takashi
H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
title H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
title_full H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
title_fullStr H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
title_full_unstemmed H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
title_short H(2)O(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
title_sort h(2)o(2)-dependent substrate oxidation by an engineered diiron site in a bacterial hemerythrin
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947700/
https://www.ncbi.nlm.nih.gov/pubmed/24400317
http://dx.doi.org/10.1039/c3cc48108e
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