Cargando…

AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas

Tryptophyllins are a diverse family of amphibian peptides originally found in extracts of phyllomedusine frog skin by chemical means. Their biological activities remain obscure. Here we describe the isolation and preliminary pharmacological characterization of a novel type 2 tryptophyllin, named AcT...

Descripción completa

Detalles Bibliográficos
Autores principales: Ge, Lilin, Lyu, Peng, Zhou, Mei, Zhang, Huiling, Wan, Yuantai, Li, Bin, Li, Renjie, Wang, Lei, Chen, Tianbao, Shaw, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947715/
https://www.ncbi.nlm.nih.gov/pubmed/24693226
http://dx.doi.org/10.1155/2014/158546
_version_ 1782306709758279680
author Ge, Lilin
Lyu, Peng
Zhou, Mei
Zhang, Huiling
Wan, Yuantai
Li, Bin
Li, Renjie
Wang, Lei
Chen, Tianbao
Shaw, Chris
author_facet Ge, Lilin
Lyu, Peng
Zhou, Mei
Zhang, Huiling
Wan, Yuantai
Li, Bin
Li, Renjie
Wang, Lei
Chen, Tianbao
Shaw, Chris
author_sort Ge, Lilin
collection PubMed
description Tryptophyllins are a diverse family of amphibian peptides originally found in extracts of phyllomedusine frog skin by chemical means. Their biological activities remain obscure. Here we describe the isolation and preliminary pharmacological characterization of a novel type 2 tryptophyllin, named AcT-2, from the skin secretion of the red-eyed leaf frog, Agalychnis callidryas. The peptide was initially identified during smooth muscle pharmacological screening of skin secretion HPLC fractions and the unique primary structure—GMRPPWF-NH(2)—was established by both Edman degradation and electrospray MS/MS fragmentation sequencing. A. cDNA encoding the biosynthetic precursor of AcT-2 was successfully cloned from a skin secretion-derived cDNA library by means of RACE PCR and this contained an open-reading frame consisting of 62 amino acid residues with a single AcT-2 encoding sequence located towards the C-terminus. A synthetic replicate of AcT-2 was found to relax arterial smooth muscle (EC(50) = 5.1 nM) and to contract rat urinary bladder smooth muscle (EC(50) = 9.3 μM). The peptide could also inhibit the growth of the microorganisms, Staphylococcus aureus, (MIC = 256 mg/L) Escherichia coli (MIC = 512 mg/L), and Candida albicans (128 mg/L). AcT-2 is thus the first amphibian skin tryptophyllin found to possess both myotropic and antimicrobial activities.
format Online
Article
Text
id pubmed-3947715
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-39477152014-04-01 AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas Ge, Lilin Lyu, Peng Zhou, Mei Zhang, Huiling Wan, Yuantai Li, Bin Li, Renjie Wang, Lei Chen, Tianbao Shaw, Chris ScientificWorldJournal Research Article Tryptophyllins are a diverse family of amphibian peptides originally found in extracts of phyllomedusine frog skin by chemical means. Their biological activities remain obscure. Here we describe the isolation and preliminary pharmacological characterization of a novel type 2 tryptophyllin, named AcT-2, from the skin secretion of the red-eyed leaf frog, Agalychnis callidryas. The peptide was initially identified during smooth muscle pharmacological screening of skin secretion HPLC fractions and the unique primary structure—GMRPPWF-NH(2)—was established by both Edman degradation and electrospray MS/MS fragmentation sequencing. A. cDNA encoding the biosynthetic precursor of AcT-2 was successfully cloned from a skin secretion-derived cDNA library by means of RACE PCR and this contained an open-reading frame consisting of 62 amino acid residues with a single AcT-2 encoding sequence located towards the C-terminus. A synthetic replicate of AcT-2 was found to relax arterial smooth muscle (EC(50) = 5.1 nM) and to contract rat urinary bladder smooth muscle (EC(50) = 9.3 μM). The peptide could also inhibit the growth of the microorganisms, Staphylococcus aureus, (MIC = 256 mg/L) Escherichia coli (MIC = 512 mg/L), and Candida albicans (128 mg/L). AcT-2 is thus the first amphibian skin tryptophyllin found to possess both myotropic and antimicrobial activities. Hindawi Publishing Corporation 2014-02-13 /pmc/articles/PMC3947715/ /pubmed/24693226 http://dx.doi.org/10.1155/2014/158546 Text en Copyright © 2014 Lilin Ge et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ge, Lilin
Lyu, Peng
Zhou, Mei
Zhang, Huiling
Wan, Yuantai
Li, Bin
Li, Renjie
Wang, Lei
Chen, Tianbao
Shaw, Chris
AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas
title AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas
title_full AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas
title_fullStr AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas
title_full_unstemmed AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas
title_short AcT-2: A Novel Myotropic and Antimicrobial Type 2 Tryptophyllin from the Skin Secretion of the Central American Red-Eyed Leaf Frog, Agalychnis callidryas
title_sort act-2: a novel myotropic and antimicrobial type 2 tryptophyllin from the skin secretion of the central american red-eyed leaf frog, agalychnis callidryas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947715/
https://www.ncbi.nlm.nih.gov/pubmed/24693226
http://dx.doi.org/10.1155/2014/158546
work_keys_str_mv AT gelilin act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT lyupeng act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT zhoumei act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT zhanghuiling act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT wanyuantai act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT libin act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT lirenjie act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT wanglei act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT chentianbao act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas
AT shawchris act2anovelmyotropicandantimicrobialtype2tryptophyllinfromtheskinsecretionofthecentralamericanredeyedleaffrogagalychniscallidryas