Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population

This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1, GSTT1, GSTP1, GCLM, and GCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n = 88), exposed to MeHg from fish consumption, were enrolled in...

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Autores principales: de Oliveira, Andréia Ávila Soares, de Souza, Marilesia Ferreira, Lengert, André van Helvoort, de Oliveira, Marcelo Tempesta, Camargo, Rossana Batista de Oliveira Godoy, Braga, Gilberto Úbida Leite, Cólus, Ilce Mara de Syllos, Barbosa, Fernando, Barcelos, Gustavo Rafael Mazzaron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947838/
https://www.ncbi.nlm.nih.gov/pubmed/24696865
http://dx.doi.org/10.1155/2014/940952
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author de Oliveira, Andréia Ávila Soares
de Souza, Marilesia Ferreira
Lengert, André van Helvoort
de Oliveira, Marcelo Tempesta
Camargo, Rossana Batista de Oliveira Godoy
Braga, Gilberto Úbida Leite
Cólus, Ilce Mara de Syllos
Barbosa, Fernando
Barcelos, Gustavo Rafael Mazzaron
author_facet de Oliveira, Andréia Ávila Soares
de Souza, Marilesia Ferreira
Lengert, André van Helvoort
de Oliveira, Marcelo Tempesta
Camargo, Rossana Batista de Oliveira Godoy
Braga, Gilberto Úbida Leite
Cólus, Ilce Mara de Syllos
Barbosa, Fernando
Barcelos, Gustavo Rafael Mazzaron
author_sort de Oliveira, Andréia Ávila Soares
collection PubMed
description This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1, GSTT1, GSTP1, GCLM, and GCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n = 88), exposed to MeHg from fish consumption, were enrolled in the study. Hg species in the plasma compartment were determined by LC-ICP-MS, whereas genotyping was performed by PCR assays. Mean total Hg levels in plasma (THgP) and whole blood (THgB) were 10 ± 4.2 and 37 ± 21, whereas mean evels of plasmatic MeHg (MeHgP), inorganic Hg (IHgP), and HgP/HgB were 4.3 ± 2.9, 5.8 ± 2.3 µg/L, and 0.33 ± 0.15, respectively. GSTM1 and GCLC polymorphisms influence THgP and MeHgP (multivariate analyses, P < 0.050). Null homozygotes for GSTM1 showed higher THgP and MeHgP levels compared to subjects with GSTM1 (THgP β = 0.22, P = 0.035; MeHgP β = 0.30, P = 0.050) and persons carrying at least one T allele for GCLC had significant higher MeHgP (β = 0.59, P = 0.046). Also, polymorphic GCLM subjects had lower THgP/THgB than those with the nonvariant genotype. Taken together, data of this study suggest that GSH-related polymorphisms may change the metabolism of MeHg by modifying the distribution of mercury species iin plasma compartment and the HgP/HgB partitioning.
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spelling pubmed-39478382014-04-02 Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population de Oliveira, Andréia Ávila Soares de Souza, Marilesia Ferreira Lengert, André van Helvoort de Oliveira, Marcelo Tempesta Camargo, Rossana Batista de Oliveira Godoy Braga, Gilberto Úbida Leite Cólus, Ilce Mara de Syllos Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron Biomed Res Int Research Article This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1, GSTT1, GSTP1, GCLM, and GCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n = 88), exposed to MeHg from fish consumption, were enrolled in the study. Hg species in the plasma compartment were determined by LC-ICP-MS, whereas genotyping was performed by PCR assays. Mean total Hg levels in plasma (THgP) and whole blood (THgB) were 10 ± 4.2 and 37 ± 21, whereas mean evels of plasmatic MeHg (MeHgP), inorganic Hg (IHgP), and HgP/HgB were 4.3 ± 2.9, 5.8 ± 2.3 µg/L, and 0.33 ± 0.15, respectively. GSTM1 and GCLC polymorphisms influence THgP and MeHgP (multivariate analyses, P < 0.050). Null homozygotes for GSTM1 showed higher THgP and MeHgP levels compared to subjects with GSTM1 (THgP β = 0.22, P = 0.035; MeHgP β = 0.30, P = 0.050) and persons carrying at least one T allele for GCLC had significant higher MeHgP (β = 0.59, P = 0.046). Also, polymorphic GCLM subjects had lower THgP/THgB than those with the nonvariant genotype. Taken together, data of this study suggest that GSH-related polymorphisms may change the metabolism of MeHg by modifying the distribution of mercury species iin plasma compartment and the HgP/HgB partitioning. Hindawi Publishing Corporation 2014 2014-02-18 /pmc/articles/PMC3947838/ /pubmed/24696865 http://dx.doi.org/10.1155/2014/940952 Text en Copyright © 2014 Andréia Ávila Soares de Oliveira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
de Oliveira, Andréia Ávila Soares
de Souza, Marilesia Ferreira
Lengert, André van Helvoort
de Oliveira, Marcelo Tempesta
Camargo, Rossana Batista de Oliveira Godoy
Braga, Gilberto Úbida Leite
Cólus, Ilce Mara de Syllos
Barbosa, Fernando
Barcelos, Gustavo Rafael Mazzaron
Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
title Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
title_full Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
title_fullStr Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
title_full_unstemmed Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
title_short Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
title_sort genetic polymorphisms in glutathione (gsh-) related genes affect the plasmatic hg/whole blood hg partitioning and the distribution between inorganic and methylmercury levels in plasma collected from a fish-eating population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947838/
https://www.ncbi.nlm.nih.gov/pubmed/24696865
http://dx.doi.org/10.1155/2014/940952
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