Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population
This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1, GSTT1, GSTP1, GCLM, and GCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n = 88), exposed to MeHg from fish consumption, were enrolled in...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947838/ https://www.ncbi.nlm.nih.gov/pubmed/24696865 http://dx.doi.org/10.1155/2014/940952 |
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author | de Oliveira, Andréia Ávila Soares de Souza, Marilesia Ferreira Lengert, André van Helvoort de Oliveira, Marcelo Tempesta Camargo, Rossana Batista de Oliveira Godoy Braga, Gilberto Úbida Leite Cólus, Ilce Mara de Syllos Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron |
author_facet | de Oliveira, Andréia Ávila Soares de Souza, Marilesia Ferreira Lengert, André van Helvoort de Oliveira, Marcelo Tempesta Camargo, Rossana Batista de Oliveira Godoy Braga, Gilberto Úbida Leite Cólus, Ilce Mara de Syllos Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron |
author_sort | de Oliveira, Andréia Ávila Soares |
collection | PubMed |
description | This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1, GSTT1, GSTP1, GCLM, and GCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n = 88), exposed to MeHg from fish consumption, were enrolled in the study. Hg species in the plasma compartment were determined by LC-ICP-MS, whereas genotyping was performed by PCR assays. Mean total Hg levels in plasma (THgP) and whole blood (THgB) were 10 ± 4.2 and 37 ± 21, whereas mean evels of plasmatic MeHg (MeHgP), inorganic Hg (IHgP), and HgP/HgB were 4.3 ± 2.9, 5.8 ± 2.3 µg/L, and 0.33 ± 0.15, respectively. GSTM1 and GCLC polymorphisms influence THgP and MeHgP (multivariate analyses, P < 0.050). Null homozygotes for GSTM1 showed higher THgP and MeHgP levels compared to subjects with GSTM1 (THgP β = 0.22, P = 0.035; MeHgP β = 0.30, P = 0.050) and persons carrying at least one T allele for GCLC had significant higher MeHgP (β = 0.59, P = 0.046). Also, polymorphic GCLM subjects had lower THgP/THgB than those with the nonvariant genotype. Taken together, data of this study suggest that GSH-related polymorphisms may change the metabolism of MeHg by modifying the distribution of mercury species iin plasma compartment and the HgP/HgB partitioning. |
format | Online Article Text |
id | pubmed-3947838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39478382014-04-02 Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population de Oliveira, Andréia Ávila Soares de Souza, Marilesia Ferreira Lengert, André van Helvoort de Oliveira, Marcelo Tempesta Camargo, Rossana Batista de Oliveira Godoy Braga, Gilberto Úbida Leite Cólus, Ilce Mara de Syllos Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron Biomed Res Int Research Article This study aims to evaluate the effects of polymorphisms in glutathione (GSH-) related genes (GSTM1, GSTT1, GSTP1, GCLM, and GCLC) in the distribution of Hg in the blood compartments in humans exposed to methylmercury (MeHg). Subjects (n = 88), exposed to MeHg from fish consumption, were enrolled in the study. Hg species in the plasma compartment were determined by LC-ICP-MS, whereas genotyping was performed by PCR assays. Mean total Hg levels in plasma (THgP) and whole blood (THgB) were 10 ± 4.2 and 37 ± 21, whereas mean evels of plasmatic MeHg (MeHgP), inorganic Hg (IHgP), and HgP/HgB were 4.3 ± 2.9, 5.8 ± 2.3 µg/L, and 0.33 ± 0.15, respectively. GSTM1 and GCLC polymorphisms influence THgP and MeHgP (multivariate analyses, P < 0.050). Null homozygotes for GSTM1 showed higher THgP and MeHgP levels compared to subjects with GSTM1 (THgP β = 0.22, P = 0.035; MeHgP β = 0.30, P = 0.050) and persons carrying at least one T allele for GCLC had significant higher MeHgP (β = 0.59, P = 0.046). Also, polymorphic GCLM subjects had lower THgP/THgB than those with the nonvariant genotype. Taken together, data of this study suggest that GSH-related polymorphisms may change the metabolism of MeHg by modifying the distribution of mercury species iin plasma compartment and the HgP/HgB partitioning. Hindawi Publishing Corporation 2014 2014-02-18 /pmc/articles/PMC3947838/ /pubmed/24696865 http://dx.doi.org/10.1155/2014/940952 Text en Copyright © 2014 Andréia Ávila Soares de Oliveira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article de Oliveira, Andréia Ávila Soares de Souza, Marilesia Ferreira Lengert, André van Helvoort de Oliveira, Marcelo Tempesta Camargo, Rossana Batista de Oliveira Godoy Braga, Gilberto Úbida Leite Cólus, Ilce Mara de Syllos Barbosa, Fernando Barcelos, Gustavo Rafael Mazzaron Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population |
title | Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population |
title_full | Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population |
title_fullStr | Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population |
title_full_unstemmed | Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population |
title_short | Genetic Polymorphisms in Glutathione (GSH-) Related Genes Affect the Plasmatic Hg/Whole Blood Hg Partitioning and the Distribution between Inorganic and Methylmercury Levels in Plasma Collected from a Fish-Eating Population |
title_sort | genetic polymorphisms in glutathione (gsh-) related genes affect the plasmatic hg/whole blood hg partitioning and the distribution between inorganic and methylmercury levels in plasma collected from a fish-eating population |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947838/ https://www.ncbi.nlm.nih.gov/pubmed/24696865 http://dx.doi.org/10.1155/2014/940952 |
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