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Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women

AIM: Elevated levels of sterol regulatory element-binding protein-1 (SREBP-1) have been found in endometrial cancer (EC), suggesting that it is essential to the development of EC. Obesity and diabetes have been established as known risk factors of EC, while SREBF-1 gene polymorphisms have also been...

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Autores principales: Qiu, Chun-Ping, Lv, Qing-Tao, Dongol, Samina, Wang, Chenguang, Jiang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948687/
https://www.ncbi.nlm.nih.gov/pubmed/24614076
http://dx.doi.org/10.1371/journal.pone.0090491
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author Qiu, Chun-Ping
Lv, Qing-Tao
Dongol, Samina
Wang, Chenguang
Jiang, Jie
author_facet Qiu, Chun-Ping
Lv, Qing-Tao
Dongol, Samina
Wang, Chenguang
Jiang, Jie
author_sort Qiu, Chun-Ping
collection PubMed
description AIM: Elevated levels of sterol regulatory element-binding protein-1 (SREBP-1) have been found in endometrial cancer (EC), suggesting that it is essential to the development of EC. Obesity and diabetes have been established as known risk factors of EC, while SREBF-1 gene polymorphisms have also been found to be associated with obesity and type II diabetes. Therefore, we hypothesize that single nucleotide polymorphism (SNP) in SREBF-1 gene may be associated with increased risk of EC. METHOD: We analyzed the sequence of SREBF-1 in tissue samples from 30 EC cases and 6 benign controls using high throughput method. Based on the primary results, we selected one SNP (rs2297508) as a genetic marker to conduct a hospital-based case-control study with 139 EC cases and 129 benign controls. The samples were examined under the microscope to determine their histopathology prior to the SNP analysis using RT-PCR. RESULTS: Through sequence analysis, we found 10 SNPs of SREBF-1 associated with EC, including 3 new SNPs. Fourteen percent of EC showed the rs2297508 SNP with C allele, while only 7% had the C allele was present in benign controls (p = 0.027, OR = 1.983). Additionally, the C allele was associated with cancer differentiation (p<0.05) and the depth of myometrial invasion (p<0.05). CONCLUSION: Our study indicates that SNP (rs2297508) of SREBF-1 may serve as a genetic predisposition factor for the development of EC and screening of such genetic marker may be helpful in its early detection.
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spelling pubmed-39486872014-03-13 Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women Qiu, Chun-Ping Lv, Qing-Tao Dongol, Samina Wang, Chenguang Jiang, Jie PLoS One Research Article AIM: Elevated levels of sterol regulatory element-binding protein-1 (SREBP-1) have been found in endometrial cancer (EC), suggesting that it is essential to the development of EC. Obesity and diabetes have been established as known risk factors of EC, while SREBF-1 gene polymorphisms have also been found to be associated with obesity and type II diabetes. Therefore, we hypothesize that single nucleotide polymorphism (SNP) in SREBF-1 gene may be associated with increased risk of EC. METHOD: We analyzed the sequence of SREBF-1 in tissue samples from 30 EC cases and 6 benign controls using high throughput method. Based on the primary results, we selected one SNP (rs2297508) as a genetic marker to conduct a hospital-based case-control study with 139 EC cases and 129 benign controls. The samples were examined under the microscope to determine their histopathology prior to the SNP analysis using RT-PCR. RESULTS: Through sequence analysis, we found 10 SNPs of SREBF-1 associated with EC, including 3 new SNPs. Fourteen percent of EC showed the rs2297508 SNP with C allele, while only 7% had the C allele was present in benign controls (p = 0.027, OR = 1.983). Additionally, the C allele was associated with cancer differentiation (p<0.05) and the depth of myometrial invasion (p<0.05). CONCLUSION: Our study indicates that SNP (rs2297508) of SREBF-1 may serve as a genetic predisposition factor for the development of EC and screening of such genetic marker may be helpful in its early detection. Public Library of Science 2014-03-10 /pmc/articles/PMC3948687/ /pubmed/24614076 http://dx.doi.org/10.1371/journal.pone.0090491 Text en © 2014 Qiu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Qiu, Chun-Ping
Lv, Qing-Tao
Dongol, Samina
Wang, Chenguang
Jiang, Jie
Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
title Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
title_full Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
title_fullStr Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
title_full_unstemmed Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
title_short Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
title_sort single nucleotide polymorphism of srebf-1 gene associated with an increased risk of endometrial cancer in chinese women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948687/
https://www.ncbi.nlm.nih.gov/pubmed/24614076
http://dx.doi.org/10.1371/journal.pone.0090491
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