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Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women
AIM: Elevated levels of sterol regulatory element-binding protein-1 (SREBP-1) have been found in endometrial cancer (EC), suggesting that it is essential to the development of EC. Obesity and diabetes have been established as known risk factors of EC, while SREBF-1 gene polymorphisms have also been...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948687/ https://www.ncbi.nlm.nih.gov/pubmed/24614076 http://dx.doi.org/10.1371/journal.pone.0090491 |
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author | Qiu, Chun-Ping Lv, Qing-Tao Dongol, Samina Wang, Chenguang Jiang, Jie |
author_facet | Qiu, Chun-Ping Lv, Qing-Tao Dongol, Samina Wang, Chenguang Jiang, Jie |
author_sort | Qiu, Chun-Ping |
collection | PubMed |
description | AIM: Elevated levels of sterol regulatory element-binding protein-1 (SREBP-1) have been found in endometrial cancer (EC), suggesting that it is essential to the development of EC. Obesity and diabetes have been established as known risk factors of EC, while SREBF-1 gene polymorphisms have also been found to be associated with obesity and type II diabetes. Therefore, we hypothesize that single nucleotide polymorphism (SNP) in SREBF-1 gene may be associated with increased risk of EC. METHOD: We analyzed the sequence of SREBF-1 in tissue samples from 30 EC cases and 6 benign controls using high throughput method. Based on the primary results, we selected one SNP (rs2297508) as a genetic marker to conduct a hospital-based case-control study with 139 EC cases and 129 benign controls. The samples were examined under the microscope to determine their histopathology prior to the SNP analysis using RT-PCR. RESULTS: Through sequence analysis, we found 10 SNPs of SREBF-1 associated with EC, including 3 new SNPs. Fourteen percent of EC showed the rs2297508 SNP with C allele, while only 7% had the C allele was present in benign controls (p = 0.027, OR = 1.983). Additionally, the C allele was associated with cancer differentiation (p<0.05) and the depth of myometrial invasion (p<0.05). CONCLUSION: Our study indicates that SNP (rs2297508) of SREBF-1 may serve as a genetic predisposition factor for the development of EC and screening of such genetic marker may be helpful in its early detection. |
format | Online Article Text |
id | pubmed-3948687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39486872014-03-13 Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women Qiu, Chun-Ping Lv, Qing-Tao Dongol, Samina Wang, Chenguang Jiang, Jie PLoS One Research Article AIM: Elevated levels of sterol regulatory element-binding protein-1 (SREBP-1) have been found in endometrial cancer (EC), suggesting that it is essential to the development of EC. Obesity and diabetes have been established as known risk factors of EC, while SREBF-1 gene polymorphisms have also been found to be associated with obesity and type II diabetes. Therefore, we hypothesize that single nucleotide polymorphism (SNP) in SREBF-1 gene may be associated with increased risk of EC. METHOD: We analyzed the sequence of SREBF-1 in tissue samples from 30 EC cases and 6 benign controls using high throughput method. Based on the primary results, we selected one SNP (rs2297508) as a genetic marker to conduct a hospital-based case-control study with 139 EC cases and 129 benign controls. The samples were examined under the microscope to determine their histopathology prior to the SNP analysis using RT-PCR. RESULTS: Through sequence analysis, we found 10 SNPs of SREBF-1 associated with EC, including 3 new SNPs. Fourteen percent of EC showed the rs2297508 SNP with C allele, while only 7% had the C allele was present in benign controls (p = 0.027, OR = 1.983). Additionally, the C allele was associated with cancer differentiation (p<0.05) and the depth of myometrial invasion (p<0.05). CONCLUSION: Our study indicates that SNP (rs2297508) of SREBF-1 may serve as a genetic predisposition factor for the development of EC and screening of such genetic marker may be helpful in its early detection. Public Library of Science 2014-03-10 /pmc/articles/PMC3948687/ /pubmed/24614076 http://dx.doi.org/10.1371/journal.pone.0090491 Text en © 2014 Qiu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Qiu, Chun-Ping Lv, Qing-Tao Dongol, Samina Wang, Chenguang Jiang, Jie Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women |
title | Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women |
title_full | Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women |
title_fullStr | Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women |
title_full_unstemmed | Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women |
title_short | Single Nucleotide Polymorphism of SREBF-1 Gene Associated with an Increased Risk of Endometrial Cancer in Chinese Women |
title_sort | single nucleotide polymorphism of srebf-1 gene associated with an increased risk of endometrial cancer in chinese women |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948687/ https://www.ncbi.nlm.nih.gov/pubmed/24614076 http://dx.doi.org/10.1371/journal.pone.0090491 |
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