Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

Approaches that depend on directed evolution require reliable methods to generate DNA diversity so that mutant libraries can focus on specific target regions. We took advantage of the high frequency of homologous DNA recombination in Saccharomyces cerevisiae to develop a strategy for domain mutagene...

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Detalles Bibliográficos
Autores principales: Gonzalez-Perez, David, Molina-Espeja, Patricia, Garcia-Ruiz, Eva, Alcalde, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948698/
https://www.ncbi.nlm.nih.gov/pubmed/24614282
http://dx.doi.org/10.1371/journal.pone.0090919

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948698/
https://www.ncbi.nlm.nih.gov/pubmed/24614282
http://dx.doi.org/10.1371/journal.pone.0090919

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