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Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72

A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing en...

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Autores principales: Schöner, Tim A., Fuchs, Sebastian W., Reinhold-Hurek, Barbara, Bode, Helge B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949708/
https://www.ncbi.nlm.nih.gov/pubmed/24618669
http://dx.doi.org/10.1371/journal.pone.0090922
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author Schöner, Tim A.
Fuchs, Sebastian W.
Reinhold-Hurek, Barbara
Bode, Helge B.
author_facet Schöner, Tim A.
Fuchs, Sebastian W.
Reinhold-Hurek, Barbara
Bode, Helge B.
author_sort Schöner, Tim A.
collection PubMed
description A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing enzyme from the XanB2-type producing 3- and 4-hydroxybenzoic acid and an AMP-ligase encoded by these gene clusters were characterized, that might perform the first two steps of the polyene biosynthesis. Furthermore, a detailed analysis of the already known or novel biosynthesis gene clusters involved in the biosynthesis of polyene containing pigments like arcuflavin, flexirubin and xanthomonadin revealed the presence of similar gene clusters in a wide range of bacterial taxa, suggesting that polyene and polyene-dialkylresorcinol pigments are more widespread than previously realized.
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spelling pubmed-39497082014-03-12 Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72 Schöner, Tim A. Fuchs, Sebastian W. Reinhold-Hurek, Barbara Bode, Helge B. PLoS One Research Article A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing enzyme from the XanB2-type producing 3- and 4-hydroxybenzoic acid and an AMP-ligase encoded by these gene clusters were characterized, that might perform the first two steps of the polyene biosynthesis. Furthermore, a detailed analysis of the already known or novel biosynthesis gene clusters involved in the biosynthesis of polyene containing pigments like arcuflavin, flexirubin and xanthomonadin revealed the presence of similar gene clusters in a wide range of bacterial taxa, suggesting that polyene and polyene-dialkylresorcinol pigments are more widespread than previously realized. Public Library of Science 2014-03-11 /pmc/articles/PMC3949708/ /pubmed/24618669 http://dx.doi.org/10.1371/journal.pone.0090922 Text en © 2014 Schöner et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schöner, Tim A.
Fuchs, Sebastian W.
Reinhold-Hurek, Barbara
Bode, Helge B.
Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72
title Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72
title_full Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72
title_fullStr Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72
title_full_unstemmed Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72
title_short Identification and Biosynthesis of a Novel Xanthomonadin-Dialkylresorcinol-Hybrid from Azoarcus sp. BH72
title_sort identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from azoarcus sp. bh72
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949708/
https://www.ncbi.nlm.nih.gov/pubmed/24618669
http://dx.doi.org/10.1371/journal.pone.0090922
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