Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1

In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA sa...

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Detalles Bibliográficos
Autores principales: Araújo, Cristina P., Osório, Ana Luiza A. R., Jorge, Kláudia S. G., Ramos, Carlos Alberto N., Filho, Antonio Francisco S., Vidal, Carlos Eugênio S., Roxo, Eliana, Nishibe, Christiane, Almeida, Nalvo F., Júnior, Antônio A. F., Silva, Marcio R., Neto, José Diomedes B., Cerqueira, Valíria D., Zumárraga, Martín J., Araújo, Flábio R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949733/
https://www.ncbi.nlm.nih.gov/pubmed/24618787
http://dx.doi.org/10.1371/journal.pone.0091023

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