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Laser-Assisted In Vitro Fertilization Facilitates Fertilization of Vitrified-Warmed C57BL/6 Mouse Oocytes with Fresh and Frozen-Thawed Spermatozoa, Producing Live Pups

The utility of cryopreserved mouse gametes for reproduction of transgenic mice depends on development of assisted reproductive technologies, including vitrification of unfertilized mouse oocytes. Due to hardening of the zona pellucida, spermatozoa are often unable to penetrate vitrified-warmed (V-W)...

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Detalles Bibliográficos
Autores principales:	Woods, Stephanie E., Qi, Peimin, Rosalia, Elizabeth, Chavarria, Tony, Discua, Allan, Mkandawire, John, Fox, James G., García, Alexis
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950285/ https://www.ncbi.nlm.nih.gov/pubmed/24618785 http://dx.doi.org/10.1371/journal.pone.0091892

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